Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3626 | 5' | -46.6 | NC_001650.1 | + | 26087 | 0.68 | 0.99981 |
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Target: 5'- aGGgAGAGGUUucCCCGAUC-CUggcUCUACu -3' miRNA: 3'- gCUgUCUUCAA--GGGUUAGaGA---AGAUG- -5' |
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| 3626 | 5' | -46.6 | NC_001650.1 | + | 52951 | 0.69 | 0.998856 |
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Target: 5'- gGGCcGGggGUUCCCAGacCUCUUUaaUACu -3' miRNA: 3'- gCUG-UCuuCAAGGGUUa-GAGAAG--AUG- -5' |
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| 3626 | 5' | -46.6 | NC_001650.1 | + | 39311 | 0.69 | 0.998608 |
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Target: 5'- aGcACcGAAGUU-UCGGUCUCUUCUGCa -3' miRNA: 3'- gC-UGuCUUCAAgGGUUAGAGAAGAUG- -5' |
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| 3626 | 5' | -46.6 | NC_001650.1 | + | 156341 | 0.72 | 0.990154 |
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Target: 5'- gGGCAGggGgugccucagCCCAGUCaUCUUgUGCu -3' miRNA: 3'- gCUGUCuuCaa-------GGGUUAG-AGAAgAUG- -5' |
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| 3626 | 5' | -46.6 | NC_001650.1 | + | 103745 | 0.75 | 0.948803 |
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Target: 5'- -cACGGAGGUUCCCuggccAUC-CUUCUACu -3' miRNA: 3'- gcUGUCUUCAAGGGu----UAGaGAAGAUG- -5' |
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| 3626 | 5' | -46.6 | NC_001650.1 | + | 39486 | 1.13 | 0.016625 |
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Target: 5'- aCGACAGAAGUUCCCAAUCUCUUCUACa -3' miRNA: 3'- -GCUGUCUUCAAGGGUUAGAGAAGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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