Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3627 | 5' | -51.7 | NC_001650.1 | + | 46520 | 0.66 | 0.989258 |
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Target: 5'- cCGGCGGcgaCCGGGcgCCCUGGcUAUUa -3' miRNA: 3'- uGUCGCCa--GGUCCaaGGGAUU-AUAGa -5' |
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| 3627 | 5' | -51.7 | NC_001650.1 | + | 87906 | 0.67 | 0.984423 |
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Target: 5'- aAUAGCuGGUCgGGGcgagaCCUGAUAUCa -3' miRNA: 3'- -UGUCG-CCAGgUCCaag--GGAUUAUAGa -5' |
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| 3627 | 5' | -51.7 | NC_001650.1 | + | 81141 | 0.67 | 0.98249 |
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Target: 5'- -gAGCaGGuUCCAGGUgccgUCCCUGAcAUCc -3' miRNA: 3'- ugUCG-CC-AGGUCCA----AGGGAUUaUAGa -5' |
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| 3627 | 5' | -51.7 | NC_001650.1 | + | 115923 | 0.67 | 0.978089 |
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Target: 5'- aGCAGUGGccUCCAGGaaCCUguuuAUGUCa -3' miRNA: 3'- -UGUCGCC--AGGUCCaaGGGau--UAUAGa -5' |
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| 3627 | 5' | -51.7 | NC_001650.1 | + | 101380 | 0.68 | 0.970031 |
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Target: 5'- gACGGUGGUCCAGGacauaUUCaCCUGcu-UCa -3' miRNA: 3'- -UGUCGCCAGGUCC-----AAG-GGAUuauAGa -5' |
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| 3627 | 5' | -51.7 | NC_001650.1 | + | 94601 | 0.69 | 0.956262 |
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Target: 5'- uCAGCGcGUCCAGGUagCCCUc--GUCc -3' miRNA: 3'- uGUCGC-CAGGUCCAa-GGGAuuaUAGa -5' |
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| 3627 | 5' | -51.7 | NC_001650.1 | + | 117237 | 0.7 | 0.938717 |
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Target: 5'- gGCGGCGG-CCAGGUcCCCguuUGUg- -3' miRNA: 3'- -UGUCGCCaGGUCCAaGGGauuAUAga -5' |
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| 3627 | 5' | -51.7 | NC_001650.1 | + | 158663 | 0.7 | 0.92846 |
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Target: 5'- uACGGgGGUCCAGGUggcuaaCCUGAccUCg -3' miRNA: 3'- -UGUCgCCAGGUCCAag----GGAUUauAGa -5' |
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| 3627 | 5' | -51.7 | NC_001650.1 | + | 39636 | 1.07 | 0.010695 |
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Target: 5'- cACAGCGGUCCAGGUUCCCUAAUAUCUc -3' miRNA: 3'- -UGUCGCCAGGUCCAAGGGAUUAUAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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