miRNA: 3'- uagUGACGuua-UGGAGAGGAGcaagu -5'
miRNA: 3'- uaGUGACgUuAUGGAGAGGaGCAAGU- -5'
miRNA: 3'- -UAGUGa-CGUuaUGGAGa-GGAGCaagu -5'
miRNA: 3'- uagUGACGuuaUGGAGAGGAGCaagu -5'
miRNA: 3'- -UAGUGACGUUAUGGAGAGGAGCAAGU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3629 | 5' | -50.2 | NC_001650.1 | + | 142655 | 0.66 | 0.995722 |
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Target: 5'- --gGCUGCugcgGCCUCUCCUCc---- -3' miRNA: 3'- uagUGACGuua-UGGAGAGGAGcaagu -5' |
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| 3629 | 5' | -50.2 | NC_001650.1 | + | 59786 | 0.67 | 0.992221 |
|
Target: 5'- -aCGCUGgAcUACCUCUUCgUGUUCAa -3' miRNA: 3'- uaGUGACgUuAUGGAGAGGaGCAAGU- -5' |
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| 3629 | 5' | -50.2 | NC_001650.1 | + | 131787 | 0.68 | 0.986754 |
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Target: 5'- gGUCACggGCAggACCUCggCCUCGg--- -3' miRNA: 3'- -UAGUGa-CGUuaUGGAGa-GGAGCaagu -5' |
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| 3629 | 5' | -50.2 | NC_001650.1 | + | 102935 | 0.69 | 0.976246 |
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Target: 5'- --uGCUGCc--GCCUCUCCUCGg--- -3' miRNA: 3'- uagUGACGuuaUGGAGAGGAGCaagu -5' |
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| 3629 | 5' | -50.2 | NC_001650.1 | + | 40513 | 1.07 | 0.014531 |
|
Target: 5'- aAUCACUGCAAUACCUCUCCUCGUUCAc -3' miRNA: 3'- -UAGUGACGUUAUGGAGAGGAGCAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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