Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3631 | 5' | -53.1 | NC_001650.1 | + | 87231 | 0.66 | 0.983211 |
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Target: 5'- gGGCCUGGAGAugcugguCUUCUCCcUGA--GCu -3' miRNA: 3'- -UCGGAUCUCU-------GAAGAGGcACUagCGu -5' |
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| 3631 | 5' | -53.1 | NC_001650.1 | + | 120943 | 0.66 | 0.982424 |
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Target: 5'- gAGCCUgcugaccaccgaaAGGGACcuguucgcgcccgUCUCCGUGGUCa-- -3' miRNA: 3'- -UCGGA-------------UCUCUGa------------AGAGGCACUAGcgu -5' |
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| 3631 | 5' | -53.1 | NC_001650.1 | + | 19265 | 0.67 | 0.971274 |
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Target: 5'- gGGCCUcuguccucucaggAGucAGAgUUUUCUGUGGUCGCu -3' miRNA: 3'- -UCGGA-------------UC--UCUgAAGAGGCACUAGCGu -5' |
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| 3631 | 5' | -53.1 | NC_001650.1 | + | 109460 | 0.67 | 0.965434 |
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Target: 5'- cGGCCUggAGAGGCUgcucaggcUCUUCGUGugccacccgggGUCGCc -3' miRNA: 3'- -UCGGA--UCUCUGA--------AGAGGCAC-----------UAGCGu -5' |
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| 3631 | 5' | -53.1 | NC_001650.1 | + | 88986 | 0.68 | 0.950522 |
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Target: 5'- gGGCCUgaAGAGG----UCCGUGAUgCGCAg -3' miRNA: 3'- -UCGGA--UCUCUgaagAGGCACUA-GCGU- -5' |
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| 3631 | 5' | -53.1 | NC_001650.1 | + | 38498 | 0.68 | 0.936869 |
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Target: 5'- uGGCCacGGGGACcccgCUUgGUGAUCGCu -3' miRNA: 3'- -UCGGa-UCUCUGaa--GAGgCACUAGCGu -5' |
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| 3631 | 5' | -53.1 | NC_001650.1 | + | 162871 | 0.69 | 0.921024 |
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Target: 5'- uGCCUGGAGAggguaUUUCUCCGccAUUGCu -3' miRNA: 3'- uCGGAUCUCU-----GAAGAGGCacUAGCGu -5' |
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| 3631 | 5' | -53.1 | NC_001650.1 | + | 86278 | 0.69 | 0.909237 |
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Target: 5'- aGGCCUucuuGAGGC-UCUCgGUGAcgaCGCAg -3' miRNA: 3'- -UCGGAu---CUCUGaAGAGgCACUa--GCGU- -5' |
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| 3631 | 5' | -53.1 | NC_001650.1 | + | 40980 | 1.09 | 0.006623 |
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Target: 5'- aAGCCUAGAGACUUCUCCGUGAUCGCAc -3' miRNA: 3'- -UCGGAUCUCUGAAGAGGCACUAGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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