miRNA: 3'- ccAUCUCAAg--CGAC----------GUCUCGGUCCCA- -5'
miRNA: 3'- -CC-AUCUCAAg--CGAC----------GUCUCGGUCCCA- -5'
miRNA: 3'- -CC-AUCUCAAg--CGAC----------GUCUCGGUCCCA- -5'
miRNA: 3'- -CC-AUCUCAAg-CGACGUC----UCGGUCCCA- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 3639 | 3' | -55.7 | NC_001650.1 | + | 179860 | 0.66 | 0.935766 |
|
Target: 5'- -uUAGGGUUaaaGCUGgagcuaggguUAGGGCUAGGGUu -3' miRNA: 3'- ccAUCUCAAg--CGAC----------GUCUCGGUCCCA- -5' |
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| 3639 | 3' | -55.7 | NC_001650.1 | + | 179899 | 0.69 | 0.802378 |
|
Target: 5'- gGGuUAGGGUUaaaGCUGgagcuaggguUAGAGCUAGGGUu -3' miRNA: 3'- -CC-AUCUCAAg--CGAC----------GUCUCGGUCCCA- -5' |
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| 3639 | 3' | -55.7 | NC_001650.1 | + | 179947 | 0.68 | 0.875161 |
|
Target: 5'- gGGuUAGGGUUaaaGCUGgagcuaggguUAGGGCUAGGGUu -3' miRNA: 3'- -CC-AUCUCAAg--CGAC----------GUCUCGGUCCCA- -5' |
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| 3639 | 3' | -55.7 | NC_001650.1 | + | 180054 | 0.69 | 0.793423 |
|
Target: 5'- gGGcUAGGGUUagGCUGCAGguaaAGUUAGGGUu -3' miRNA: 3'- -CC-AUCUCAAg-CGACGUC----UCGGUCCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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