Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 3649 | 3' | -54.7 | NC_001650.1 | + | 163603 | 0.73 | 0.636592 |
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Target: 5'- cCGCCGCuGUuuggaaaauuagcAGGCUCGGGGAUGGaCUCAu -3' miRNA: 3'- -GCGGUG-UA-------------UCUGGGUCCCUAUC-GAGU- -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 80698 | 0.74 | 0.617085 |
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Target: 5'- gGCUACAcGGACCCGGGGAgcgacGGCg-- -3' miRNA: 3'- gCGGUGUaUCUGGGUCCCUa----UCGagu -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 165116 | 0.74 | 0.617085 |
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Target: 5'- gGCCGCGgugGGGCCCGGGGGguggggUGGCg-- -3' miRNA: 3'- gCGGUGUa--UCUGGGUCCCU------AUCGagu -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 49497 | 0.75 | 0.526066 |
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Target: 5'- gGCCAgCuccAGGCCCGGGGGcAGCUCGu -3' miRNA: 3'- gCGGU-Gua-UCUGGGUCCCUaUCGAGU- -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 152526 | 0.76 | 0.458902 |
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Target: 5'- aGCCACcacgcaaaAGACCCAGGGAUGGUa-- -3' miRNA: 3'- gCGGUGua------UCUGGGUCCCUAUCGagu -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 47741 | 1.09 | 0.004261 |
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Target: 5'- gCGCCACAUAGACCCAGGGAUAGCUCAg -3' miRNA: 3'- -GCGGUGUAUCUGGGUCCCUAUCGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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