Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 3649 | 3' | -54.7 | NC_001650.1 | + | 155102 | 0.66 | 0.94084 |
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Target: 5'- cCGCCAcCGUGGAgacggccgugggguUgCGGGGGUAGUUCc -3' miRNA: 3'- -GCGGU-GUAUCU--------------GgGUCCCUAUCGAGu -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 149709 | 0.66 | 0.940371 |
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Target: 5'- gGCCGCucucuggagauGAUUCAGGGGUAGCg-- -3' miRNA: 3'- gCGGUGuau--------CUGGGUCCCUAUCGagu -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 35868 | 0.66 | 0.954966 |
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Target: 5'- gCGCCGCGggGGACCUggaguuucacgcGGGGcgGGCg-- -3' miRNA: 3'- -GCGGUGUa-UCUGGG------------UCCCuaUCGagu -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 78351 | 0.66 | 0.954966 |
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Target: 5'- gGCCAUccuGACagAGGGggGGCUCAg -3' miRNA: 3'- gCGGUGuauCUGggUCCCuaUCGAGU- -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 1767 | 0.66 | 0.954966 |
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Target: 5'- gGCCAUggAGGCCCuguGGGAgguGCcCAu -3' miRNA: 3'- gCGGUGuaUCUGGGu--CCCUau-CGaGU- -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 35672 | 0.65 | 0.961963 |
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Target: 5'- aGCCAgggguuCGUGGACCUccuggccGGGG--GGCUCAc -3' miRNA: 3'- gCGGU------GUAUCUGGG-------UCCCuaUCGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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