Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 3649 | 3' | -54.7 | NC_001650.1 | + | 167992 | 0.72 | 0.678567 |
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Target: 5'- aGCCAauggGGGCCCGGGGGUGGgCcCAa -3' miRNA: 3'- gCGGUgua-UCUGGGUCCCUAUC-GaGU- -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 168177 | 0.66 | 0.950954 |
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Target: 5'- aGCCAauggGGGCCCGGGG--GGCg-- -3' miRNA: 3'- gCGGUgua-UCUGGGUCCCuaUCGagu -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 168474 | 0.66 | 0.954966 |
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Target: 5'- gGCCAUggAGGCCCuguGGGAgguGCcCAu -3' miRNA: 3'- gCGGUGuaUCUGGGu--CCCUau-CGaGU- -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 173217 | 0.69 | 0.862419 |
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Target: 5'- gGCC-CAauagGGACCCAGGGggAGUUg- -3' miRNA: 3'- gCGGuGUa---UCUGGGUCCCuaUCGAgu -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 173665 | 0.67 | 0.937512 |
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Target: 5'- uGCCAgGcAGACUaGGGGGU-GCUCAc -3' miRNA: 3'- gCGGUgUaUCUGGgUCCCUAuCGAGU- -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 174478 | 0.66 | 0.954966 |
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Target: 5'- uGCCGgGcAGACUaGGGGGU-GCUCAa -3' miRNA: 3'- gCGGUgUaUCUGGgUCCCUAuCGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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