Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 3649 | 3' | -54.7 | NC_001650.1 | + | 155102 | 0.66 | 0.94084 |
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Target: 5'- cCGCCAcCGUGGAgacggccgugggguUgCGGGGGUAGUUCc -3' miRNA: 3'- -GCGGU-GUAUCU--------------GgGUCCCUAUCGAGu -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 149709 | 0.66 | 0.940371 |
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Target: 5'- gGCCGCucucuggagauGAUUCAGGGGUAGCg-- -3' miRNA: 3'- gCGGUGuau--------CUGGGUCCCUAUCGagu -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 66866 | 0.67 | 0.937512 |
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Target: 5'- gGCCGCGggAGGCgCCGGGGGcgggGGCg-- -3' miRNA: 3'- gCGGUGUa-UCUG-GGUCCCUa---UCGagu -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 81193 | 0.67 | 0.932556 |
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Target: 5'- gGCCACGUAG-CCCAcGuGGAU-GUUCu -3' miRNA: 3'- gCGGUGUAUCuGGGU-C-CCUAuCGAGu -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 105819 | 0.67 | 0.927359 |
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Target: 5'- gGCCugAUAGAUCCGGGcuacaggggugaGAUAaagcugaucguGCUCAa -3' miRNA: 3'- gCGGugUAUCUGGGUCC------------CUAU-----------CGAGU- -5' |
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| 3649 | 3' | -54.7 | NC_001650.1 | + | 80698 | 0.74 | 0.617085 |
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Target: 5'- gGCUACAcGGACCCGGGGAgcgacGGCg-- -3' miRNA: 3'- gCGGUGUaUCUGGGUCCCUa----UCGagu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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