Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3657 | 3' | -56.9 | NC_001650.1 | + | 136622 | 0.71 | 0.655229 |
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Target: 5'- --cCGcCCGGGGGCgucggGgcGCCCUGAGg -3' miRNA: 3'- caaGC-GGCCCUCGga---CauUGGGACUC- -5' |
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| 3657 | 3' | -56.9 | NC_001650.1 | + | 121855 | 0.71 | 0.645155 |
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Target: 5'- -gUCGUCcucgGGGGGCCUGggcaggaaGAUCCUGAGg -3' miRNA: 3'- caAGCGG----CCCUCGGACa-------UUGGGACUC- -5' |
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| 3657 | 3' | -56.9 | NC_001650.1 | + | 127184 | 0.71 | 0.645155 |
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Target: 5'- -cUCGugaaCCGGGAGCUguUGACCCUGAc -3' miRNA: 3'- caAGC----GGCCCUCGGacAUUGGGACUc -5' |
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| 3657 | 3' | -56.9 | NC_001650.1 | + | 35306 | 0.71 | 0.63507 |
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Target: 5'- ---gGCCaGGGccacacGCCUGUGGCCCUGAc -3' miRNA: 3'- caagCGG-CCCu-----CGGACAUUGGGACUc -5' |
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| 3657 | 3' | -56.9 | NC_001650.1 | + | 11379 | 0.72 | 0.614901 |
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Target: 5'- ---gGCgCGGGGGCCUGUAaagaGCgCUGGGg -3' miRNA: 3'- caagCG-GCCCUCGGACAU----UGgGACUC- -5' |
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| 3657 | 3' | -56.9 | NC_001650.1 | + | 178086 | 0.72 | 0.614901 |
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Target: 5'- ---gGCgCGGGGGCCUGUAaagaGCgCUGGGg -3' miRNA: 3'- caagCG-GCCCUCGGACAU----UGgGACUC- -5' |
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| 3657 | 3' | -56.9 | NC_001650.1 | + | 124371 | 0.72 | 0.574771 |
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Target: 5'- -aUCGCCGGGGGUCgc--GCCCaUGAGc -3' miRNA: 3'- caAGCGGCCCUCGGacauUGGG-ACUC- -5' |
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| 3657 | 3' | -56.9 | NC_001650.1 | + | 24057 | 0.75 | 0.450484 |
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Target: 5'- -gUCGgggaCGGGGGCCUGUGggcgGCCCUGGc -3' miRNA: 3'- caAGCg---GCCCUCGGACAU----UGGGACUc -5' |
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| 3657 | 3' | -56.9 | NC_001650.1 | + | 140236 | 0.87 | 0.081903 |
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Target: 5'- --cCGCCGGGAGCaUGUAACCCUGGGc -3' miRNA: 3'- caaGCGGCCCUCGgACAUUGGGACUC- -5' |
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| 3657 | 3' | -56.9 | NC_001650.1 | + | 54428 | 1.07 | 0.003643 |
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Target: 5'- gGUUCGCCGGGAGCCUGUAACCCUGAGc -3' miRNA: 3'- -CAAGCGGCCCUCGGACAUUGGGACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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