Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 366 | 3' | -58.7 | AC_000011.1 | + | 9678 | 0.66 | 0.400783 |
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Target: 5'- cGGUUcugagacgGCGGAUGGUGGCgagGAGCa- -3' miRNA: 3'- uCCAG--------CGCCUACUACCGggaCUCGag -5' |
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| 366 | 3' | -58.7 | AC_000011.1 | + | 14284 | 0.67 | 0.355864 |
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Target: 5'- aAGG-CGCGGGUGAUGGUCUcccGCa- -3' miRNA: 3'- -UCCaGCGCCUACUACCGGGacuCGag -5' |
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| 366 | 3' | -58.7 | AC_000011.1 | + | 10025 | 0.68 | 0.29164 |
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Target: 5'- -aGUUGaCGGucUGGUGGCCCggacgcacGAGCUCg -3' miRNA: 3'- ucCAGC-GCCu-ACUACCGGGa-------CUCGAG- -5' |
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| 366 | 3' | -58.7 | AC_000011.1 | + | 22438 | 0.68 | 0.29164 |
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Target: 5'- cGGUUcUGGGUGAucuUGGCCCggucgGGGUUCu -3' miRNA: 3'- uCCAGcGCCUACU---ACCGGGa----CUCGAG- -5' |
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| 366 | 3' | -58.7 | AC_000011.1 | + | 8816 | 0.72 | 0.161826 |
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Target: 5'- gAGGUCGuUGGA-GAUgcGGCCCaUGAGCUg -3' miRNA: 3'- -UCCAGC-GCCUaCUA--CCGGG-ACUCGAg -5' |
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| 366 | 3' | -58.7 | AC_000011.1 | + | 21202 | 1.08 | 0.00027 |
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Target: 5'- cAGGUCGCGGAUGAUGGCCCUGAGCUCc -3' miRNA: 3'- -UCCAGCGCCUACUACCGGGACUCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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