Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 367 | 5' | -60.7 | AC_000011.1 | + | 13713 | 0.66 | 0.333177 |
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Target: 5'- -aGCaGCGUGUugGAC-UUGGGUGgGa -3' miRNA: 3'- gaCGaCGCACGugCUGcGGCCCACgC- -5' |
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| 367 | 5' | -60.7 | AC_000011.1 | + | 16337 | 0.66 | 0.325212 |
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Target: 5'- -cGCccGCGccacUGCACGcgcCGCCGGGggGCGa -3' miRNA: 3'- gaCGa-CGC----ACGUGCu--GCGGCCCa-CGC- -5' |
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| 367 | 5' | -60.7 | AC_000011.1 | + | 11149 | 0.67 | 0.287519 |
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Target: 5'- -aGCUGCG-GCGCGGC-CUggaccgaaagaGGGUGCu -3' miRNA: 3'- gaCGACGCaCGUGCUGcGG-----------CCCACGc -5' |
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| 367 | 5' | -60.7 | AC_000011.1 | + | 16552 | 0.67 | 0.287519 |
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Target: 5'- gUGCUGCcgaccgcgGCGCcGCGCCGGGgguucaaGCGc -3' miRNA: 3'- gACGACGca------CGUGcUGCGGCCCa------CGC- -5' |
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| 367 | 5' | -60.7 | AC_000011.1 | + | 5226 | 0.69 | 0.211205 |
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Target: 5'- gUGC-GCGccggGCugGGCGCUugcgaGGGUGCGc -3' miRNA: 3'- gACGaCGCa---CGugCUGCGG-----CCCACGC- -5' |
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| 367 | 5' | -60.7 | AC_000011.1 | + | 13102 | 0.7 | 0.181965 |
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Target: 5'- aUGUUGCGcgcggucaugucgaGCGCGGCGCUGGGgGUGg -3' miRNA: 3'- gACGACGCa-------------CGUGCUGCGGCCCaCGC- -5' |
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| 367 | 5' | -60.7 | AC_000011.1 | + | 2131 | 0.73 | 0.112821 |
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Target: 5'- gCUGCUGC-UGCugGCGACGuuGGcGUGCc -3' miRNA: 3'- -GACGACGcACG--UGCUGCggCC-CACGc -5' |
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| 367 | 5' | -60.7 | AC_000011.1 | + | 15990 | 0.74 | 0.090045 |
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Target: 5'- --aCUGgGUGCGCGACGCCGccaccGGUGUGc -3' miRNA: 3'- gacGACgCACGUGCUGCGGC-----CCACGC- -5' |
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| 367 | 5' | -60.7 | AC_000011.1 | + | 22432 | 1.07 | 0.000232 |
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Target: 5'- gCUGCUGCGUGCACGACGCCGGGUGCGc -3' miRNA: 3'- -GACGACGCACGUGCUGCGGCCCACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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