Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3686 | 5' | -62.6 | NC_001650.1 | + | 106263 | 0.7 | 0.452311 |
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Target: 5'- gCGACGGCCCCUC----GGUGcCUCGCa -3' miRNA: 3'- -GCUGUCGGGGAGagcgCCGC-GAGCGa -5' |
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| 3686 | 5' | -62.6 | NC_001650.1 | + | 50929 | 0.7 | 0.434302 |
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Target: 5'- gGGCAGCCCCcuccccuUCUCuCGGCGCggggGCUu -3' miRNA: 3'- gCUGUCGGGG-------AGAGcGCCGCGag--CGA- -5' |
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| 3686 | 5' | -62.6 | NC_001650.1 | + | 66722 | 0.7 | 0.410131 |
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Target: 5'- gGACGGUgUUguagCUCGCGGCGC-CGCa -3' miRNA: 3'- gCUGUCGgGGa---GAGCGCCGCGaGCGa -5' |
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| 3686 | 5' | -62.6 | NC_001650.1 | + | 142188 | 0.7 | 0.410131 |
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Target: 5'- uGACGGCCaCC-CaCGCGGCGCU-GCUc -3' miRNA: 3'- gCUGUCGG-GGaGaGCGCCGCGAgCGA- -5' |
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| 3686 | 5' | -62.6 | NC_001650.1 | + | 101606 | 0.73 | 0.280192 |
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Target: 5'- gGGCAGCCCCUCUuccccaucccCGaGGCGCUC-CUg -3' miRNA: 3'- gCUGUCGGGGAGA----------GCgCCGCGAGcGA- -5' |
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| 3686 | 5' | -62.6 | NC_001650.1 | + | 54898 | 0.75 | 0.208287 |
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Target: 5'- gGAUucugAGCCCCcggCUCGCGGC-CUCGCUc -3' miRNA: 3'- gCUG----UCGGGGa--GAGCGCCGcGAGCGA- -5' |
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| 3686 | 5' | -62.6 | NC_001650.1 | + | 108772 | 0.98 | 0.005276 |
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Target: 5'- gCGACAGCCCC-CUCGCGGCGCUCGCUu -3' miRNA: 3'- -GCUGUCGGGGaGAGCGCCGCGAGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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