Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 369 | 5' | -58.9 | AC_000011.1 | + | 5297 | 0.66 | 0.367871 |
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Target: 5'- cGGcgcCCUGC---GCGUCGGCCAGGuaGCa -3' miRNA: 3'- aCCa--GGACGuccUGCAGUCGGUCC--UG- -5' |
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| 369 | 5' | -58.9 | AC_000011.1 | + | 15136 | 0.66 | 0.359133 |
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Target: 5'- gGGUCUUGCAucAUGUCGGgCAGcGACc -3' miRNA: 3'- aCCAGGACGUccUGCAGUCgGUC-CUG- -5' |
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| 369 | 5' | -58.9 | AC_000011.1 | + | 34248 | 0.67 | 0.350541 |
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Target: 5'- gUGGUUCcGCAGGA--UCAuCCGGGGCu -3' miRNA: 3'- -ACCAGGaCGUCCUgcAGUcGGUCCUG- -5' |
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| 369 | 5' | -58.9 | AC_000011.1 | + | 28333 | 0.67 | 0.342096 |
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Target: 5'- cGGUgC-GCGGGGagcCGUCAGCaccgGGGACa -3' miRNA: 3'- aCCAgGaCGUCCU---GCAGUCGg---UCCUG- -5' |
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| 369 | 5' | -58.9 | AC_000011.1 | + | 11130 | 0.68 | 0.293051 |
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Target: 5'- cGGUUCcacGCGGGGCGggagcugcggcgCGGCCuGGACc -3' miRNA: 3'- aCCAGGa--CGUCCUGCa-----------GUCGGuCCUG- -5' |
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| 369 | 5' | -58.9 | AC_000011.1 | + | 12641 | 0.69 | 0.245121 |
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Target: 5'- aGGgCCUGUGGGGCGUgcaGGCCccggucgGGGACc -3' miRNA: 3'- aCCaGGACGUCCUGCAg--UCGG-------UCCUG- -5' |
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| 369 | 5' | -58.9 | AC_000011.1 | + | 8674 | 0.7 | 0.22102 |
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Target: 5'- gGGUCCcGUGGccuucacccagaGGCGUCAGauCCAGGACg -3' miRNA: 3'- aCCAGGaCGUC------------CUGCAGUC--GGUCCUG- -5' |
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| 369 | 5' | -58.9 | AC_000011.1 | + | 23113 | 1.1 | 0.000192 |
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Target: 5'- gUGGUCCUGCAGGACGUCAGCCAGGACa -3' miRNA: 3'- -ACCAGGACGUCCUGCAGUCGGUCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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