Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3703 | 3' | -53.2 | NC_001650.1 | + | 161050 | 0.72 | 0.816676 |
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Target: 5'- cCCUGgaGGGCGCGGUGGgccuguaggaggccgUCccgCACCAc -3' miRNA: 3'- -GGACgaUCUGUGCCACC---------------AGaa-GUGGU- -5' |
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| 3703 | 3' | -53.2 | NC_001650.1 | + | 106962 | 0.73 | 0.738652 |
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Target: 5'- gCCUGCaGGAguCGGUGGUCUcgUGCCc -3' miRNA: 3'- -GGACGaUCUguGCCACCAGAa-GUGGu -5' |
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| 3703 | 3' | -53.2 | NC_001650.1 | + | 36410 | 0.74 | 0.729804 |
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Target: 5'- --aGCUGGACACGGUGGcCggggagugcgugggCGCCAa -3' miRNA: 3'- ggaCGAUCUGUGCCACCaGaa------------GUGGU- -5' |
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| 3703 | 3' | -53.2 | NC_001650.1 | + | 66741 | 0.74 | 0.708904 |
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Target: 5'- aCCaGCUGGugACGGUGGggggcgCUcCGCCGc -3' miRNA: 3'- -GGaCGAUCugUGCCACCa-----GAaGUGGU- -5' |
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| 3703 | 3' | -53.2 | NC_001650.1 | + | 61923 | 0.77 | 0.535978 |
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Target: 5'- uUUGUgGGACACgGGUGGUCUaUCGCCAa -3' miRNA: 3'- gGACGaUCUGUG-CCACCAGA-AGUGGU- -5' |
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| 3703 | 3' | -53.2 | NC_001650.1 | + | 120547 | 0.79 | 0.422629 |
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Target: 5'- aCCUGCaGGGCGCGGUGGggCUUCugACCu -3' miRNA: 3'- -GGACGaUCUGUGCCACCa-GAAG--UGGu -5' |
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| 3703 | 3' | -53.2 | NC_001650.1 | + | 83852 | 0.89 | 0.120714 |
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Target: 5'- gCCUGCUGGACGCGGUGGUCUUU-CUg -3' miRNA: 3'- -GGACGAUCUGUGCCACCAGAAGuGGu -5' |
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| 3703 | 3' | -53.2 | NC_001650.1 | + | 97133 | 1.11 | 0.005178 |
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Target: 5'- cCCUGCUAGACACGGUGGUCUUCACCAu -3' miRNA: 3'- -GGACGAUCUGUGCCACCAGAAGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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