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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 372 | 3' | -56.1 | AC_000011.1 | + | 13868 | 0.67 | 0.50292 |
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Target: 5'- --aGUaUGAUGAgGCGUgcguacccggaggGUCCUCCUCCc -3' miRNA: 3'- cuaCGaACUACU-CGCG-------------CAGGAGGGGG- -5' |
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| 372 | 3' | -56.1 | AC_000011.1 | + | 652 | 0.68 | 0.393413 |
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Target: 5'- aAUGCcaUGAUGGGCGaCGacccUCCggagCCCCCc -3' miRNA: 3'- cUACGa-ACUACUCGC-GC----AGGa---GGGGG- -5' |
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| 372 | 3' | -56.1 | AC_000011.1 | + | 17357 | 0.7 | 0.299822 |
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Target: 5'- cGAUGaugggGAUcAGCGCGggcaggaagCCUCCCCCc -3' miRNA: 3'- -CUACgaa--CUAcUCGCGCa--------GGAGGGGG- -5' |
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| 372 | 3' | -56.1 | AC_000011.1 | + | 13253 | 0.71 | 0.26195 |
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Target: 5'- cGAUGUggacagcAGCGUGUUCUCCCCCc -3' miRNA: 3'- -CUACGaacuac-UCGCGCAGGAGGGGG- -5' |
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| 372 | 3' | -56.1 | AC_000011.1 | + | 23748 | 1.11 | 0.000341 |
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Target: 5'- aGAUGCUUGAUGAGCGCGUCCUCCCCCc -3' miRNA: 3'- -CUACGAACUACUCGCGCAGGAGGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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