Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3726 | 3' | -59.6 | NC_001650.1 | + | 57739 | 0.69 | 0.587643 |
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Target: 5'- cCCGGcCUCGCggggugGGCGacgGGGGAGUa-- -3' miRNA: 3'- uGGUC-GAGCGa-----CCGCg--CCCCUCAaag -5' |
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| 3726 | 3' | -59.6 | NC_001650.1 | + | 66789 | 0.69 | 0.577762 |
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Target: 5'- -aCAGCaaGCUGuccCGgGGGGAGUUUCa -3' miRNA: 3'- ugGUCGagCGACc--GCgCCCCUCAAAG- -5' |
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| 3726 | 3' | -59.6 | NC_001650.1 | + | 21665 | 0.7 | 0.529025 |
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Target: 5'- gACCAGCggggaCGaC-GGCGCGGGGGGg--- -3' miRNA: 3'- -UGGUCGa----GC-GaCCGCGCCCCUCaaag -5' |
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| 3726 | 3' | -59.6 | NC_001650.1 | + | 29035 | 0.7 | 0.51945 |
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Target: 5'- uACCcGCaCGCccucggGGCGCGGGGGGUg-- -3' miRNA: 3'- -UGGuCGaGCGa-----CCGCGCCCCUCAaag -5' |
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| 3726 | 3' | -59.6 | NC_001650.1 | + | 120539 | 0.71 | 0.481883 |
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Target: 5'- cGCCGGCgaccUGCagGGCGCGGuGGGGcUUCu -3' miRNA: 3'- -UGGUCGa---GCGa-CCGCGCC-CCUCaAAG- -5' |
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| 3726 | 3' | -59.6 | NC_001650.1 | + | 115942 | 0.72 | 0.436871 |
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Target: 5'- aACCAGCa-GCUGGUGCgccgGGGGAGgagCg -3' miRNA: 3'- -UGGUCGagCGACCGCG----CCCCUCaaaG- -5' |
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| 3726 | 3' | -59.6 | NC_001650.1 | + | 88472 | 1.08 | 0.001837 |
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Target: 5'- cACCAGCUCGCUGGCGCGGGGAGUUUCc -3' miRNA: 3'- -UGGUCGAGCGACCGCGCCCCUCAAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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