Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 373 | 3' | -55.3 | AC_000011.1 | + | 29038 | 0.67 | 0.514499 |
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Target: 5'- cUGGGUGggGUGGGUUgaguGCUauuguUGACCa- -3' miRNA: 3'- -AUCCGCuuUACCCAGu---CGA-----GCUGGca -5' |
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| 373 | 3' | -55.3 | AC_000011.1 | + | 6830 | 0.68 | 0.461204 |
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Target: 5'- gUGGGCGug--GGG-CAGUcCGACCGa -3' miRNA: 3'- -AUCCGCuuuaCCCaGUCGaGCUGGCa -5' |
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| 373 | 3' | -55.3 | AC_000011.1 | + | 7706 | 0.68 | 0.450893 |
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Target: 5'- aGGGCGAGAUcgaGGGcgAGCUCGacgaGCCGg -3' miRNA: 3'- aUCCGCUUUA---CCCagUCGAGC----UGGCa -5' |
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| 373 | 3' | -55.3 | AC_000011.1 | + | 8476 | 0.68 | 0.44071 |
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Target: 5'- cGGGCGGcagGGG-CGGCUCGGggcCCGg -3' miRNA: 3'- aUCCGCUuuaCCCaGUCGAGCU---GGCa -5' |
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| 373 | 3' | -55.3 | AC_000011.1 | + | 31398 | 0.7 | 0.338067 |
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Target: 5'- -cGGgGAGcAUGGGUCgcuguGGCUCGGCCu- -3' miRNA: 3'- auCCgCUU-UACCCAG-----UCGAGCUGGca -5' |
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| 373 | 3' | -55.3 | AC_000011.1 | + | 24241 | 1.06 | 0.000815 |
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Target: 5'- gUAGGCGAAAUGGGUCAGCUCGACCGUg -3' miRNA: 3'- -AUCCGCUUUACCCAGUCGAGCUGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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