Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 374 | 5' | -54.8 | AC_000011.1 | + | 12233 | 0.68 | 0.50749 |
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Target: 5'- uCC-UGGCCAUCGUGAac--GCGCUGg -3' miRNA: 3'- uGGuGCCGGUAGUACUcaaaCGCGGC- -5' |
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| 374 | 5' | -54.8 | AC_000011.1 | + | 10165 | 0.68 | 0.486008 |
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Target: 5'- --aGCGGCCAUCGcucGGUggcggggGCGCCGg -3' miRNA: 3'- uggUGCCGGUAGUac-UCAaa-----CGCGGC- -5' |
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| 374 | 5' | -54.8 | AC_000011.1 | + | 11288 | 0.68 | 0.464968 |
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Target: 5'- gGCCGCGGCCA-CGUGcGcgcGCGCgGg -3' miRNA: 3'- -UGGUGCCGGUaGUACuCaaaCGCGgC- -5' |
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| 374 | 5' | -54.8 | AC_000011.1 | + | 18516 | 0.68 | 0.464968 |
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Target: 5'- gGCUGCGGUcgguggucaCAUCGUGcGUggGCGCCa -3' miRNA: 3'- -UGGUGCCG---------GUAGUACuCAaaCGCGGc -5' |
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| 374 | 5' | -54.8 | AC_000011.1 | + | 11583 | 0.7 | 0.385976 |
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Target: 5'- cUgGCGGCCAUCAacuucucggugcUGAGUUUGgGCa- -3' miRNA: 3'- uGgUGCCGGUAGU------------ACUCAAACgCGgc -5' |
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| 374 | 5' | -54.8 | AC_000011.1 | + | 17365 | 0.7 | 0.358723 |
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Target: 5'- cGCCGCGGCgAUgAUGGGgaucaGCGCgGg -3' miRNA: 3'- -UGGUGCCGgUAgUACUCaaa--CGCGgC- -5' |
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| 374 | 5' | -54.8 | AC_000011.1 | + | 8853 | 0.71 | 0.341316 |
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Target: 5'- cUCGCGGCCAcCGUgGAGc--GCGCCGg -3' miRNA: 3'- uGGUGCCGGUaGUA-CUCaaaCGCGGC- -5' |
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| 374 | 5' | -54.8 | AC_000011.1 | + | 10967 | 0.72 | 0.292819 |
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Target: 5'- gGCCGCGGCgGUCGUGGcuGgaagUUGCuGCUGg -3' miRNA: 3'- -UGGUGCCGgUAGUACU--Ca---AACG-CGGC- -5' |
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| 374 | 5' | -54.8 | AC_000011.1 | + | 10923 | 0.75 | 0.179624 |
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Target: 5'- cGCCGCGGCCGcCGUGAGcg-GgGCUGg -3' miRNA: 3'- -UGGUGCCGGUaGUACUCaaaCgCGGC- -5' |
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| 374 | 5' | -54.8 | AC_000011.1 | + | 24451 | 1.08 | 0.000735 |
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Target: 5'- gACCACGGCCAUCAUGAGUUUGCGCCGc -3' miRNA: 3'- -UGGUGCCGGUAGUACUCAAACGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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