miRNA: 3'- -CCGGCUAGG-GCaagCGCCugAUacua -5'
miRNA: 3'- -CCGGCUAGgGCAAGCGC---CugA-UACUA- -5'
miRNA: 3'- -CCGGCUAGGGCAAGCGCCUGAUACUA- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 377 | 5' | -54.9 | AC_000011.1 | + | 5655 | 0.66 | 0.62017 |
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Target: 5'- gGGCCGGUCCuCGa--GCGGugUGc--- -3' miRNA: 3'- -CCGGCUAGG-GCaagCGCCugAUacua -5' |
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| 377 | 5' | -54.9 | AC_000011.1 | + | 34589 | 0.69 | 0.446329 |
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Target: 5'- cGGCUGAUCgUGUUCGCGaccGugUcAUGAUg -3' miRNA: 3'- -CCGGCUAGgGCAAGCGC---CugA-UACUA- -5' |
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| 377 | 5' | -54.9 | AC_000011.1 | + | 33827 | 1.08 | 0.000833 |
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Target: 5'- cGGCCGAUCCCGUUCGCGGACUAUGAUu -3' miRNA: 3'- -CCGGCUAGGGCAAGCGCCUGAUACUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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