Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 378 | 5' | -58.1 | AC_000011.1 | + | 26752 | 0.66 | 0.407387 |
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Target: 5'- cAGCugGAcuUCGGCCuggGCGUCUCGCgGa- -3' miRNA: 3'- -UUGugCU--AGUCGG---CGCAGGGCGgUag -5' |
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| 378 | 5' | -58.1 | AC_000011.1 | + | 15803 | 0.66 | 0.40644 |
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Target: 5'- uGGCGCGGgcguacCGGCgCGCGUCCgccaccaCGCUGUCg -3' miRNA: 3'- -UUGUGCUa-----GUCG-GCGCAGG-------GCGGUAG- -5' |
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| 378 | 5' | -58.1 | AC_000011.1 | + | 11556 | 0.66 | 0.386865 |
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Target: 5'- gAGCGCGggCuGCCGCuGUCCgagaagcuggcgGCCAUCa -3' miRNA: 3'- -UUGUGCuaGuCGGCG-CAGGg-----------CGGUAG- -5' |
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| 378 | 5' | -58.1 | AC_000011.1 | + | 33977 | 0.68 | 0.304062 |
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Target: 5'- cGCAUGcgCuGCUGCG-CCCGCCGc- -3' miRNA: 3'- uUGUGCuaGuCGGCGCaGGGCGGUag -5' |
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| 378 | 5' | -58.1 | AC_000011.1 | + | 10663 | 0.7 | 0.240638 |
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Target: 5'- -uCGCGGUCGGCCGCuUUCCGCg--- -3' miRNA: 3'- uuGUGCUAGUCGGCGcAGGGCGguag -5' |
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| 378 | 5' | -58.1 | AC_000011.1 | + | 34638 | 1.08 | 0.000329 |
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Target: 5'- gAACACGAUCAGCCGCGUCCCGCCAUCa -3' miRNA: 3'- -UUGUGCUAGUCGGCGCAGGGCGGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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