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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 379 | 5' | -59 | AC_000011.1 | + | 11273 | 0.67 | 0.301174 |
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Target: 5'- -cGCgc-GCGCgGGGCUgauccccgucagcucGUCCGCCUCg -3' miRNA: 3'- cuCGaaaCGCGaCCUGA---------------CGGGCGGAG- -5' |
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| 379 | 5' | -59 | AC_000011.1 | + | 5200 | 0.67 | 0.282445 |
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Target: 5'- uGGC--UGCGCUGGACgcccaucgucuggUGCCCuaCUCc -3' miRNA: 3'- cUCGaaACGCGACCUG-------------ACGGGcgGAG- -5' |
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| 379 | 5' | -59 | AC_000011.1 | + | 8839 | 0.68 | 0.248433 |
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Target: 5'- uGAGCUgcgagaagGCGUUca--UGCCCGCCUCg -3' miRNA: 3'- -CUCGAaa------CGCGAccugACGGGCGGAG- -5' |
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| 379 | 5' | -59 | AC_000011.1 | + | 13083 | 0.72 | 0.147407 |
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Target: 5'- cGAGCgcgGCGCUGGGggugGCCC-CCUCc -3' miRNA: 3'- -CUCGaaaCGCGACCUga--CGGGcGGAG- -5' |
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| 379 | 5' | -59 | AC_000011.1 | + | 1006 | 1.09 | 0.000193 |
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Target: 5'- aGAGCUUUGCGCUGGACUGCCCGCCUCu -3' miRNA: 3'- -CUCGAAACGCGACCUGACGGGCGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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