Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 380 | 5' | -58 | AC_000011.1 | + | 8981 | 0.66 | 0.474557 |
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Target: 5'- uGGAGcucaaccucgCCCAGGuggucauGCGCGCCGGCGaUCc -3' miRNA: 3'- -CCUCuca-------GGGUCC-------CGUGCGGUUGC-AG- -5' |
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| 380 | 5' | -58 | AC_000011.1 | + | 28285 | 0.66 | 0.427825 |
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Target: 5'- -cGGGGUCCCcGGGC-UGCCAcACGg- -3' miRNA: 3'- ccUCUCAGGGuCCCGuGCGGU-UGCag -5' |
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| 380 | 5' | -58 | AC_000011.1 | + | 9550 | 0.67 | 0.40317 |
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Target: 5'- -cGGAGUCCCuacGGGGCAauugauaagaugcauCGUCAGCGcCc -3' miRNA: 3'- ccUCUCAGGG---UCCCGU---------------GCGGUUGCaG- -5' |
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| 380 | 5' | -58 | AC_000011.1 | + | 12524 | 0.68 | 0.36356 |
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Target: 5'- ---uGGcCCCGGGGCACGUUGGCGg- -3' miRNA: 3'- ccucUCaGGGUCCCGUGCGGUUGCag -5' |
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| 380 | 5' | -58 | AC_000011.1 | + | 25839 | 0.68 | 0.362691 |
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Target: 5'- aGGAGcggcagggauacaAGUCCUggcgGGGGCacaaaaACGCCAuCGUCu -3' miRNA: 3'- -CCUC-------------UCAGGG----UCCCG------UGCGGUuGCAG- -5' |
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| 380 | 5' | -58 | AC_000011.1 | + | 10976 | 0.68 | 0.354941 |
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Target: 5'- uGGAGAGggCgaGGGGCuggcGCGCCugggGGCGUCg -3' miRNA: 3'- -CCUCUCa-GggUCCCG----UGCGG----UUGCAG- -5' |
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| 380 | 5' | -58 | AC_000011.1 | + | 8284 | 0.71 | 0.236876 |
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Target: 5'- aGGAGuuuUUCCAGGGCGCGCgGGagGUCc -3' miRNA: 3'- -CCUCuc-AGGGUCCCGUGCGgUUg-CAG- -5' |
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| 380 | 5' | -58 | AC_000011.1 | + | 9445 | 0.73 | 0.166825 |
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Target: 5'- gGGGGGGUCCCcguuGGGCagggagaggGCGCUGACGa- -3' miRNA: 3'- -CCUCUCAGGGu---CCCG---------UGCGGUUGCag -5' |
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| 380 | 5' | -58 | AC_000011.1 | + | 2054 | 1.12 | 0.000191 |
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Target: 5'- aGGAGAGUCCCAGGGCACGCCAACGUCg -3' miRNA: 3'- -CCUCUCAGGGUCCCGUGCGGUUGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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