Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3802 | 3' | -43.6 | NC_001650.1 | + | 32963 | 0.66 | 1 |
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Target: 5'- gCugUCacaaagUACGGUGUGggGUUGggGCAg -3' miRNA: 3'- -GugAGaa----AUGUCACACuuUAGUa-CGU- -5' |
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| 3802 | 3' | -43.6 | NC_001650.1 | + | 8703 | 0.66 | 1 |
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Target: 5'- cCACUCUgguggGCGGUGaGuuGUgCAUGCGu -3' miRNA: 3'- -GUGAGAaa---UGUCACaCuuUA-GUACGU- -5' |
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| 3802 | 3' | -43.6 | NC_001650.1 | + | 175410 | 0.66 | 1 |
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Target: 5'- cCACUCUgguggGCGGUGaGuuGUgCAUGCGu -3' miRNA: 3'- -GUGAGAaa---UGUCACaCuuUA-GUACGU- -5' |
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| 3802 | 3' | -43.6 | NC_001650.1 | + | 90711 | 0.68 | 0.999994 |
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Target: 5'- cCGCUCUUgUACAGg--GggGUC-UGCAg -3' miRNA: 3'- -GUGAGAA-AUGUCacaCuuUAGuACGU- -5' |
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| 3802 | 3' | -43.6 | NC_001650.1 | + | 161793 | 0.99 | 0.185114 |
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Target: 5'- cCAUUCUUUACAGUGUGAAAUCAUGUg -3' miRNA: 3'- -GUGAGAAAUGUCACACUUUAGUACGu -5' |
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| 3802 | 3' | -43.6 | NC_001650.1 | + | 22388 | 1.11 | 0.04123 |
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Target: 5'- cCACUCUUUACAGUGUGAAAUCAUGCAg -3' miRNA: 3'- -GUGAGAAAUGUCACACUUUAGUACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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