Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 381 | 3' | -48.5 | AC_000011.1 | + | 14811 | 0.66 | 0.926292 |
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Target: 5'- cUCUACCgaggUCAGGGgcgauaauuuUGCAAGCGc -3' miRNA: 3'- -AGGUGGaaaaGGUCCCau--------ACGUUUGU- -5' |
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| 381 | 3' | -48.5 | AC_000011.1 | + | 974 | 0.67 | 0.865703 |
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Target: 5'- -aCGCagcgagCCAGGGaGUGCAAGCGc -3' miRNA: 3'- agGUGgaaaa-GGUCCCaUACGUUUGU- -5' |
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| 381 | 3' | -48.5 | AC_000011.1 | + | 36282 | 0.68 | 0.856747 |
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Target: 5'- gUCACCggUUUCUGGGcGUGUGCuGGGCGu -3' miRNA: 3'- aGGUGGa-AAAGGUCC-CAUACG-UUUGU- -5' |
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| 381 | 3' | -48.5 | AC_000011.1 | + | 7579 | 0.68 | 0.838005 |
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Target: 5'- aUCCACgg---CCAGGGcgguuUGCAGACGg -3' miRNA: 3'- -AGGUGgaaaaGGUCCCau---ACGUUUGU- -5' |
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| 381 | 3' | -48.5 | AC_000011.1 | + | 906 | 0.73 | 0.552481 |
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Target: 5'- cUCUGCCgggUCUAgGGGUAUGCAGugAa -3' miRNA: 3'- -AGGUGGaaaAGGU-CCCAUACGUUugU- -5' |
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| 381 | 3' | -48.5 | AC_000011.1 | + | 5787 | 1.1 | 0.002313 |
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Target: 5'- gUCCACCUUUUCCAGGGUAUGCAAACAc -3' miRNA: 3'- -AGGUGGAAAAGGUCCCAUACGUUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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