Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 381 | 5' | -51.2 | AC_000011.1 | + | 12088 | 0.66 | 0.829484 |
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Target: 5'- -cCGcCCGCAUCgCGGGAucaggAGGUGGcUGu -3' miRNA: 3'- gaGCaGGUGUAG-GUCCU-----UCCACUaAC- -5' |
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| 381 | 5' | -51.2 | AC_000011.1 | + | 27083 | 0.67 | 0.743124 |
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Target: 5'- aCUCGUCCGgGUaguggccgggggagCCGGaGAAGG-GGUUGa -3' miRNA: 3'- -GAGCAGGUgUA--------------GGUC-CUUCCaCUAAC- -5' |
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| 381 | 5' | -51.2 | AC_000011.1 | + | 33568 | 0.68 | 0.712781 |
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Target: 5'- ---uUCCAUGUCCuGGGAGGGUGGa-- -3' miRNA: 3'- gagcAGGUGUAGG-UCCUUCCACUaac -5' |
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| 381 | 5' | -51.2 | AC_000011.1 | + | 14788 | 0.7 | 0.585129 |
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Target: 5'- -gCGUCgACAUCCAGGAgcgcGGG-GAUg- -3' miRNA: 3'- gaGCAGgUGUAGGUCCU----UCCaCUAac -5' |
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| 381 | 5' | -51.2 | AC_000011.1 | + | 12917 | 0.71 | 0.539234 |
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Target: 5'- gUCGUCCugGcCCAGGGcgcggcucacguGGGUGAUc- -3' miRNA: 3'- gAGCAGGugUaGGUCCU------------UCCACUAac -5' |
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| 381 | 5' | -51.2 | AC_000011.1 | + | 17098 | 0.72 | 0.451774 |
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Target: 5'- aCUC-UCCGCA-CCAGGGcggcagGGGUGGUUGc -3' miRNA: 3'- -GAGcAGGUGUaGGUCCU------UCCACUAAC- -5' |
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| 381 | 5' | -51.2 | AC_000011.1 | + | 31484 | 0.79 | 0.195575 |
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Target: 5'- -cCGUCCAUGUCCAGGAgaAGGUcGUUGa -3' miRNA: 3'- gaGCAGGUGUAGGUCCU--UCCAcUAAC- -5' |
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| 381 | 5' | -51.2 | AC_000011.1 | + | 5822 | 1.1 | 0.001311 |
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Target: 5'- cCUCGUCCACAUCCAGGAAGGUGAUUGg -3' miRNA: 3'- -GAGCAGGUGUAGGUCCUUCCACUAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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