Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 382 | 5' | -58.6 | AC_000011.1 | + | 5504 | 0.67 | 0.411162 |
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Target: 5'- aCUCCACGa--GCCagGUGAGGUCGggcUGGu -3' miRNA: 3'- -GAGGUGCcacCGG--CGCUCCAGCa--ACC- -5' |
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| 382 | 5' | -58.6 | AC_000011.1 | + | 14177 | 0.68 | 0.341284 |
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Target: 5'- -aCCACGGUGGUCaggaaguuGCuGuGGUCGUucUGGu -3' miRNA: 3'- gaGGUGCCACCGG--------CG-CuCCAGCA--ACC- -5' |
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| 382 | 5' | -58.6 | AC_000011.1 | + | 15754 | 0.68 | 0.325211 |
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Target: 5'- gUCCACGGUGGagacgggCGCGGcGG-CGggGGu -3' miRNA: 3'- gAGGUGCCACCg------GCGCU-CCaGCaaCC- -5' |
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| 382 | 5' | -58.6 | AC_000011.1 | + | 10975 | 0.78 | 0.075904 |
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Target: 5'- -cUCACGGcGGCCGCGGcGGUCGUggcUGGa -3' miRNA: 3'- gaGGUGCCaCCGGCGCU-CCAGCA---ACC- -5' |
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| 382 | 5' | -58.6 | AC_000011.1 | + | 9432 | 0.8 | 0.047865 |
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Target: 5'- uCUCCA-GGUGGCCGgGGGGgucccCGUUGGg -3' miRNA: 3'- -GAGGUgCCACCGGCgCUCCa----GCAACC- -5' |
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| 382 | 5' | -58.6 | AC_000011.1 | + | 8800 | 1.1 | 0.000269 |
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Target: 5'- gCUCCACGGUGGCCGCGAGGUCGUUGGa -3' miRNA: 3'- -GAGGUGCCACCGGCGCUCCAGCAACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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