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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3876 | 3' | -48.3 | NC_001650.1 | + | 126806 | 0.66 | 0.999306 |
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Target: 5'- aGUGCCguGCGCGCCCGggccgcAGGggGUGg -3' miRNA: 3'- -UAUGG--UGUGUGGGCaua---UCUaaCACa -5' |
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| 3876 | 3' | -48.3 | NC_001650.1 | + | 44747 | 0.67 | 0.998718 |
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Target: 5'- gAUGCCACACcUuuGUcauGGUUGUGUa -3' miRNA: 3'- -UAUGGUGUGuGggCAuauCUAACACA- -5' |
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| 3876 | 3' | -48.3 | NC_001650.1 | + | 63035 | 0.68 | 0.996819 |
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Target: 5'- -cGCCggGCGCACCCGUAggucuGGUcGUGg -3' miRNA: 3'- uaUGG--UGUGUGGGCAUau---CUAaCACa -5' |
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| 3876 | 3' | -48.3 | NC_001650.1 | + | 67889 | 0.69 | 0.993615 |
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Target: 5'- -gACCGCGCACUCGUGUuuguucuugGUGUg -3' miRNA: 3'- uaUGGUGUGUGGGCAUAucuaa----CACA- -5' |
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| 3876 | 3' | -48.3 | NC_001650.1 | + | 146085 | 0.74 | 0.907807 |
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Target: 5'- -cACCACACAUCCGUAcuGAUuagGUGUa -3' miRNA: 3'- uaUGGUGUGUGGGCAUauCUAa--CACA- -5' |
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| 3876 | 3' | -48.3 | NC_001650.1 | + | 38096 | 1.05 | 0.028763 |
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Target: 5'- uAUACCACACACCCGUAUAGAUUGUGUu -3' miRNA: 3'- -UAUGGUGUGUGGGCAUAUCUAACACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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