Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3880 | 5' | -53.6 | NC_001650.1 | + | 80949 | 0.67 | 0.947864 |
Target: 5'- aGCUCGCCUGGA-CAugUg-GACGGa -3' miRNA: 3'- aUGAGUGGACCUaGUugGgaCUGCUa -5' |
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3880 | 5' | -53.6 | NC_001650.1 | + | 72554 | 0.68 | 0.911222 |
Target: 5'- gAC-CACCUGGAgaacggGACCCUGAgGGa -3' miRNA: 3'- aUGaGUGGACCUag----UUGGGACUgCUa -5' |
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3880 | 5' | -53.6 | NC_001650.1 | + | 55618 | 0.71 | 0.812573 |
Target: 5'- -cUUCACCUGGGUCAcggGCUCggcggGGCGGUg -3' miRNA: 3'- auGAGUGGACCUAGU---UGGGa----CUGCUA- -5' |
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3880 | 5' | -53.6 | NC_001650.1 | + | 79684 | 0.71 | 0.794429 |
Target: 5'- aGCUgCGCCUGGGUCAggGCCUgcgucucgGGCGAg -3' miRNA: 3'- aUGA-GUGGACCUAGU--UGGGa-------CUGCUa -5' |
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3880 | 5' | -53.6 | NC_001650.1 | + | 146192 | 0.74 | 0.62334 |
Target: 5'- -gUUCGCCaGGAUCu-CCCUGACGGUg -3' miRNA: 3'- auGAGUGGaCCUAGuuGGGACUGCUA- -5' |
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3880 | 5' | -53.6 | NC_001650.1 | + | 40675 | 1.05 | 0.008988 |
Target: 5'- aUACUCACCUGGAUCAACCCUGACGAUg -3' miRNA: 3'- -AUGAGUGGACCUAGUUGGGACUGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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