Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 389 | 3' | -58.5 | AC_000011.1 | + | 20726 | 0.66 | 0.426835 |
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Target: 5'- gCUaCCUC-GCGCCCAccaugcGCCaggGCCAGCc -3' miRNA: 3'- gGAaGGAGuCGUGGGU------CGGg--CGGUUG- -5' |
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| 389 | 3' | -58.5 | AC_000011.1 | + | 30364 | 0.66 | 0.426835 |
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Target: 5'- gCUaCCUcCAGUGCCUucucuAGCaCCGCCAAUc -3' miRNA: 3'- gGAaGGA-GUCGUGGG-----UCG-GGCGGUUG- -5' |
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| 389 | 3' | -58.5 | AC_000011.1 | + | 26684 | 0.66 | 0.426835 |
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Target: 5'- aCUUCCgcgAGaCGCCCAGgCCGaaguCCAGCu -3' miRNA: 3'- gGAAGGag-UC-GUGGGUCgGGC----GGUUG- -5' |
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| 389 | 3' | -58.5 | AC_000011.1 | + | 34198 | 0.66 | 0.42105 |
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Target: 5'- --aUCCUgcggaaccacagggcCAGCaccGCCCcGCCCGCCAuGCa -3' miRNA: 3'- ggaAGGA---------------GUCG---UGGGuCGGGCGGU-UG- -5' |
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| 389 | 3' | -58.5 | AC_000011.1 | + | 5994 | 0.66 | 0.41722 |
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Target: 5'- ---aCCUCGGCACUCAGgUUGUCAGu -3' miRNA: 3'- ggaaGGAGUCGUGGGUCgGGCGGUUg -5' |
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| 389 | 3' | -58.5 | AC_000011.1 | + | 23454 | 0.66 | 0.389185 |
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Target: 5'- aCCgcCC-CGcCGCCCAGCCCcGCCAc- -3' miRNA: 3'- -GGaaGGaGUcGUGGGUCGGG-CGGUug -5' |
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| 389 | 3' | -58.5 | AC_000011.1 | + | 13520 | 0.66 | 0.398392 |
|
Target: 5'- --cUCCUCuuCGCCCAGCaagCGCgGGCg -3' miRNA: 3'- ggaAGGAGucGUGGGUCGg--GCGgUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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