Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
389 | 5' | -53.7 | AC_000011.1 | + | 32112 | 0.67 | 0.594385 |
Target: 5'- aGGGaCAGGAGGAauuugACAUCccCUUCAg -3' miRNA: 3'- -CCCgGUCCUCCUga---UGUGGuuGAAGU- -5' |
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389 | 5' | -53.7 | AC_000011.1 | + | 15864 | 0.68 | 0.549024 |
Target: 5'- aGGGCCAuGcucAGGGCggccagACGCgCGGCUUCAg -3' miRNA: 3'- -CCCGGUcC---UCCUGa-----UGUG-GUUGAAGU- -5' |
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389 | 5' | -53.7 | AC_000011.1 | + | 23070 | 0.69 | 0.472658 |
Target: 5'- aGGCCgAGGAGGAgCUGgcgGCCGuggGCUUCAg -3' miRNA: 3'- cCCGG-UCCUCCU-GAUg--UGGU---UGAAGU- -5' |
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389 | 5' | -53.7 | AC_000011.1 | + | 2734 | 0.7 | 0.462175 |
Target: 5'- gGGGUCAGGucggugugaGGGGCUGCaguuuuucaGCCAACUg-- -3' miRNA: 3'- -CCCGGUCC---------UCCUGAUG---------UGGUUGAagu -5' |
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389 | 5' | -53.7 | AC_000011.1 | + | 7275 | 0.77 | 0.16755 |
Target: 5'- uGGGagCAGGGGGGCUGCgacgauAUCGACUUCAa -3' miRNA: 3'- -CCCg-GUCCUCCUGAUG------UGGUUGAAGU- -5' |
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389 | 5' | -53.7 | AC_000011.1 | + | 12482 | 1.11 | 0.000622 |
Target: 5'- gGGGCCAGGAGGACUACACCAACUUCAu -3' miRNA: 3'- -CCCGGUCCUCCUGAUGUGGUUGAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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