miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
389 5' -53.7 AC_000011.1 + 32112 0.67 0.594385
Target:  5'- aGGGaCAGGAGGAauuugACAUCccCUUCAg -3'
miRNA:   3'- -CCCgGUCCUCCUga---UGUGGuuGAAGU- -5'
389 5' -53.7 AC_000011.1 + 15864 0.68 0.549024
Target:  5'- aGGGCCAuGcucAGGGCggccagACGCgCGGCUUCAg -3'
miRNA:   3'- -CCCGGUcC---UCCUGa-----UGUG-GUUGAAGU- -5'
389 5' -53.7 AC_000011.1 + 23070 0.69 0.472658
Target:  5'- aGGCCgAGGAGGAgCUGgcgGCCGuggGCUUCAg -3'
miRNA:   3'- cCCGG-UCCUCCU-GAUg--UGGU---UGAAGU- -5'
389 5' -53.7 AC_000011.1 + 2734 0.7 0.462175
Target:  5'- gGGGUCAGGucggugugaGGGGCUGCaguuuuucaGCCAACUg-- -3'
miRNA:   3'- -CCCGGUCC---------UCCUGAUG---------UGGUUGAagu -5'
389 5' -53.7 AC_000011.1 + 7275 0.77 0.16755
Target:  5'- uGGGagCAGGGGGGCUGCgacgauAUCGACUUCAa -3'
miRNA:   3'- -CCCg-GUCCUCCUGAUG------UGGUUGAAGU- -5'
389 5' -53.7 AC_000011.1 + 12482 1.11 0.000622
Target:  5'- gGGGCCAGGAGGACUACACCAACUUCAu -3'
miRNA:   3'- -CCCGGUCCUCCUGAUGUGGUUGAAGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.