Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 390 | 3' | -58 | AC_000011.1 | + | 23505 | 0.66 | 0.384957 |
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Target: 5'- uGGGCggcgggGCGguuaagcuuucauUCUGCUGCUGCUGCUu -3' miRNA: 3'- gCUUGag----CGC-------------GGACGACGACGACGAc -5' |
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| 390 | 3' | -58 | AC_000011.1 | + | 21467 | 0.67 | 0.350245 |
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Target: 5'- cCGcGCUCGgcCGCCUGCggGCUcuuCUGCUGc -3' miRNA: 3'- -GCuUGAGC--GCGGACGa-CGAc--GACGAC- -5' |
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| 390 | 3' | -58 | AC_000011.1 | + | 30292 | 0.68 | 0.325096 |
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Target: 5'- aCGAGCUgGCaaCCU-CUGCUGCUGUa- -3' miRNA: 3'- -GCUUGAgCGc-GGAcGACGACGACGac -5' |
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| 390 | 3' | -58 | AC_000011.1 | + | 3925 | 0.68 | 0.309089 |
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Target: 5'- cCGcGGCcCGCGUCUGCUcCUGCaGCUGa -3' miRNA: 3'- -GC-UUGaGCGCGGACGAcGACGaCGAC- -5' |
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| 390 | 3' | -58 | AC_000011.1 | + | 2144 | 0.77 | 0.074224 |
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Target: 5'- uGAucCUC-CuCCUGCUGCUGCUGCUGg -3' miRNA: 3'- gCUu-GAGcGcGGACGACGACGACGAC- -5' |
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| 390 | 3' | -58 | AC_000011.1 | + | 36144 | 0.78 | 0.063997 |
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Target: 5'- uGAcuCUUGCGCCUGUUGUgUGCUGCUGc -3' miRNA: 3'- gCUu-GAGCGCGGACGACG-ACGACGAC- -5' |
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| 390 | 3' | -58 | AC_000011.1 | + | 12703 | 1.09 | 0.000246 |
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Target: 5'- cCGAACUCGCGCCUGCUGCUGCUGCUGg -3' miRNA: 3'- -GCUUGAGCGCGGACGACGACGACGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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