Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 392 | 5' | -55.5 | AC_000011.1 | + | 23372 | 0.67 | 0.485427 |
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Target: 5'- aAGCAUGGAGaCUCAGCcau--CGCCAa -3' miRNA: 3'- -UUGUACCUC-GGGUCGuacauGCGGUc -5' |
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| 392 | 5' | -55.5 | AC_000011.1 | + | 20358 | 0.68 | 0.384319 |
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Target: 5'- gAACccGGAGCCCAGCGaGgGCGUCu- -3' miRNA: 3'- -UUGuaCCUCGGGUCGUaCaUGCGGuc -5' |
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| 392 | 5' | -55.5 | AC_000011.1 | + | 20244 | 0.69 | 0.365831 |
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Target: 5'- cGGgAUGGGGUagAGCAUGUugGCCGc -3' miRNA: 3'- -UUgUACCUCGggUCGUACAugCGGUc -5' |
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| 392 | 5' | -55.5 | AC_000011.1 | + | 8985 | 0.69 | 0.363111 |
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Target: 5'- cACGUGGAGCUCaaccucgcccagguGGuCAUGcGCGCCGGc -3' miRNA: 3'- uUGUACCUCGGG--------------UC-GUACaUGCGGUC- -5' |
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| 392 | 5' | -55.5 | AC_000011.1 | + | 9401 | 0.7 | 0.314134 |
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Target: 5'- ----cGGGGCCgCAGCGUGaagACGCCGc -3' miRNA: 3'- uuguaCCUCGG-GUCGUACa--UGCGGUc -5' |
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| 392 | 5' | -55.5 | AC_000011.1 | + | 15353 | 0.7 | 0.290434 |
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Target: 5'- cAGCAguauccggGGAGUCCAGCGcGUgaccguuacugACGCCAGa -3' miRNA: 3'- -UUGUa-------CCUCGGGUCGUaCA-----------UGCGGUC- -5' |
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| 392 | 5' | -55.5 | AC_000011.1 | + | 20163 | 0.72 | 0.209576 |
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Target: 5'- gAGCGUGGAgGCCguGU-UGUGCGCCAu -3' miRNA: 3'- -UUGUACCU-CGGguCGuACAUGCGGUc -5' |
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| 392 | 5' | -55.5 | AC_000011.1 | + | 6404 | 0.73 | 0.19265 |
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Target: 5'- gGGCAgGGGGUCCAGCAUGaccuCGUCGGg -3' miRNA: 3'- -UUGUaCCUCGGGUCGUACau--GCGGUC- -5' |
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| 392 | 5' | -55.5 | AC_000011.1 | + | 13061 | 1.05 | 0.000741 |
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Target: 5'- cAACAUGGAGCCCAGCAUGUACGCCAGc -3' miRNA: 3'- -UUGUACCUCGGGUCGUACAUGCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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