Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 398 | 3' | -50.9 | AC_000011.1 | + | 34757 | 0.66 | 0.848154 |
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Target: 5'- aGCAGAUCuaGGGCcUCAGGAgugaugaagaUCCCa -3' miRNA: 3'- aCGUCUGGcaCCUGuAGUUCUa---------AGGG- -5' |
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| 398 | 3' | -50.9 | AC_000011.1 | + | 12379 | 0.66 | 0.829484 |
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Target: 5'- cGCgAGGCCGUGGccCAgcgCGAGcgGUUCCa -3' miRNA: 3'- aCG-UCUGGCACCu-GUa--GUUC--UAAGGg -5' |
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| 398 | 3' | -50.9 | AC_000011.1 | + | 23054 | 0.66 | 0.819793 |
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Target: 5'- gGCGG-CCGUGGGCuUCAGcuacccCCCg -3' miRNA: 3'- aCGUCuGGCACCUGuAGUUcuaa--GGG- -5' |
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| 398 | 3' | -50.9 | AC_000011.1 | + | 24416 | 0.67 | 0.799757 |
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Target: 5'- cUGguGACCGUGGAgcUgGAGug-CCUg -3' miRNA: 3'- -ACguCUGGCACCUguAgUUCuaaGGG- -5' |
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| 398 | 3' | -50.9 | AC_000011.1 | + | 9587 | 0.67 | 0.757422 |
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Target: 5'- -uCAGAucCCGUGGAC-UCGAGAcgCUCa -3' miRNA: 3'- acGUCU--GGCACCUGuAGUUCUaaGGG- -5' |
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| 398 | 3' | -50.9 | AC_000011.1 | + | 7629 | 0.68 | 0.701363 |
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Target: 5'- cGCccuGGCCGUGGAUcUCuGGAcUUCCUu -3' miRNA: 3'- aCGu--CUGGCACCUGuAGuUCU-AAGGG- -5' |
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| 398 | 3' | -50.9 | AC_000011.1 | + | 33941 | 0.82 | 0.122375 |
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Target: 5'- aUGCugaGGGCCGUGGGCAUCAugcugagGGAgUCCCu -3' miRNA: 3'- -ACG---UCUGGCACCUGUAGU-------UCUaAGGG- -5' |
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| 398 | 3' | -50.9 | AC_000011.1 | + | 16738 | 1.14 | 0.000789 |
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Target: 5'- gUGCAGACCGUGGACAUCAAGAUUCCCa -3' miRNA: 3'- -ACGUCUGGCACCUGUAGUUCUAAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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