Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 398 | 5' | -54.9 | AC_000011.1 | + | 25410 | 0.66 | 0.576064 |
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Target: 5'- cUGGGugauGACGCAGGCCGAcaGCUCGg--- -3' miRNA: 3'- uACCU----UUGCGUCUGGCU--CGGGUacua -5' |
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| 398 | 5' | -54.9 | AC_000011.1 | + | 8200 | 0.66 | 0.553197 |
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Target: 5'- -aGGgcGCGCAGGCCGGagcuGUCCAgGGUc -3' miRNA: 3'- uaCCuuUGCGUCUGGCU----CGGGUaCUA- -5' |
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| 398 | 5' | -54.9 | AC_000011.1 | + | 34825 | 0.66 | 0.530609 |
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Target: 5'- cGUGGAAugGgcCAGACCcAGCCagAUGAUg -3' miRNA: 3'- -UACCUUugC--GUCUGGcUCGGg-UACUA- -5' |
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| 398 | 5' | -54.9 | AC_000011.1 | + | 16818 | 0.67 | 0.508365 |
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Target: 5'- cAUGGAggUGCAGACgGAuCCC-UGGa -3' miRNA: 3'- -UACCUuuGCGUCUGgCUcGGGuACUa -5' |
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| 398 | 5' | -54.9 | AC_000011.1 | + | 6712 | 0.67 | 0.465139 |
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Target: 5'- -aGGggGCGaGGAgccCCGGGCCCAgguUGGUg -3' miRNA: 3'- uaCCuuUGCgUCU---GGCUCGGGU---ACUA- -5' |
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| 398 | 5' | -54.9 | AC_000011.1 | + | 22298 | 0.68 | 0.444253 |
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Target: 5'- -gGGAugAACGCcGACCaGGCCCAgaUGAUg -3' miRNA: 3'- uaCCU--UUGCGuCUGGcUCGGGU--ACUA- -5' |
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| 398 | 5' | -54.9 | AC_000011.1 | + | 11473 | 0.68 | 0.434009 |
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Target: 5'- -gGGAGGCGCuGcugaauaucACCGAGCCCGaGGg -3' miRNA: 3'- uaCCUUUGCGuC---------UGGCUCGGGUaCUa -5' |
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| 398 | 5' | -54.9 | AC_000011.1 | + | 27482 | 0.7 | 0.348412 |
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Target: 5'- -cGGGAACG-AGACCGAGCuCCAg--- -3' miRNA: 3'- uaCCUUUGCgUCUGGCUCG-GGUacua -5' |
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| 398 | 5' | -54.9 | AC_000011.1 | + | 16773 | 1.03 | 0.001281 |
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Target: 5'- cAUGGAAACGCAGACCGAGCCCAUGAUc -3' miRNA: 3'- -UACCUUUGCGUCUGGCUCGGGUACUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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