Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 4025 | 3' | -58.6 | NC_001650.1 | + | 145785 | 0.69 | 0.652976 |
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Target: 5'- cGGUugcGGAGCGGCgGCGcCacAGCGgCGACa -3' miRNA: 3'- -CCA---CCUCGCCG-CGCaGa-UCGCgGUUG- -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 148045 | 1.12 | 0.001261 |
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Target: 5'- cGGUGGAGCGGCGCGUCUAGCGCCAACg -3' miRNA: 3'- -CCACCUCGCCGCGCAGAUCGCGGUUG- -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 150975 | 0.66 | 0.845464 |
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Target: 5'- gGGUGGcGGUGGCgGCGg-UGGUGgCGGCg -3' miRNA: 3'- -CCACC-UCGCCG-CGCagAUCGCgGUUG- -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 152484 | 0.68 | 0.731238 |
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Target: 5'- uGGUGGGGCauGGCGCccgcGGCGUCGAg -3' miRNA: 3'- -CCACCUCG--CCGCGcagaUCGCGGUUg -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 154474 | 0.66 | 0.837572 |
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Target: 5'- uGG-GGcGCGGC-CGcCUggGGCGCUAGCg -3' miRNA: 3'- -CCaCCuCGCCGcGCaGA--UCGCGGUUG- -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 163262 | 0.73 | 0.442256 |
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Target: 5'- gGGUGGAGCGGgGCGa--GGCGgUAGCc -3' miRNA: 3'- -CCACCUCGCCgCGCagaUCGCgGUUG- -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 166950 | 0.68 | 0.721651 |
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Target: 5'- cGGUGG-GCGGaGCuUC-AGCGUCAACc -3' miRNA: 3'- -CCACCuCGCCgCGcAGaUCGCGGUUG- -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 172794 | 0.67 | 0.812863 |
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Target: 5'- gGGUGGgcugGGUGGCGCGcggCgagAGgGCCGu- -3' miRNA: 3'- -CCACC----UCGCCGCGCa--Ga--UCgCGGUug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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