Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 4025 | 3' | -58.6 | NC_001650.1 | + | 26051 | 0.67 | 0.804307 |
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Target: 5'- uGGuUGGccuacAGCGGCG-GcCUgAGCGCCGGCc -3' miRNA: 3'- -CC-ACC-----UCGCCGCgCaGA-UCGCGGUUG- -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 25199 | 0.66 | 0.837572 |
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Target: 5'- aGUGGGGgGGCGUG-CUgaaaagggGGCGUCAc- -3' miRNA: 3'- cCACCUCgCCGCGCaGA--------UCGCGGUug -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 21173 | 0.67 | 0.777788 |
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Target: 5'- aGGcGG-GCGGCGCGgguugcgaccgUCU-GCGCCAc- -3' miRNA: 3'- -CCaCCuCGCCGCGC-----------AGAuCGCGGUug -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 20680 | 0.73 | 0.469103 |
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Target: 5'- cGGgGGGGaCGGgGgGUCUGGUGCCGGg -3' miRNA: 3'- -CCaCCUC-GCCgCgCAGAUCGCGGUUg -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 19532 | 0.67 | 0.812863 |
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Target: 5'- uGUGcAGCGGCGCGagUCggggggguugAGCGCCGc- -3' miRNA: 3'- cCACcUCGCCGCGC--AGa---------UCGCGGUug -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 19336 | 0.75 | 0.336897 |
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Target: 5'- gGGUGGcGGCGGCGCGcUCUAGUcaggGCCu-- -3' miRNA: 3'- -CCACC-UCGCCGCGC-AGAUCG----CGGuug -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 6087 | 0.67 | 0.812863 |
|
Target: 5'- gGGUGGgcugGGUGGCGCGcggCgagAGgGCCGu- -3' miRNA: 3'- -CCACC----UCGCCGCGCa--Ga--UCgCGGUug -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 243 | 0.68 | 0.721651 |
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Target: 5'- cGGUGG-GCGGaGCuUC-AGCGUCAACc -3' miRNA: 3'- -CCACCuCGCCgCGcAGaUCGCGGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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