Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 4025 | 3' | -58.6 | NC_001650.1 | + | 31249 | 0.66 | 0.837572 |
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Target: 5'- gGGUGGGGCgaagGGgGCGga-GGCcauuGCCGACg -3' miRNA: 3'- -CCACCUCG----CCgCGCagaUCG----CGGUUG- -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 111938 | 0.67 | 0.804307 |
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Target: 5'- uGGUGu-GCGGCGCGg--GGCGCauccagGACg -3' miRNA: 3'- -CCACcuCGCCGCGCagaUCGCGg-----UUG- -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 34654 | 0.66 | 0.837572 |
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Target: 5'- --gGGGGUGGUGgGUaccAGCGCCuACa -3' miRNA: 3'- ccaCCUCGCCGCgCAga-UCGCGGuUG- -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 26051 | 0.67 | 0.804307 |
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Target: 5'- uGGuUGGccuacAGCGGCG-GcCUgAGCGCCGGCc -3' miRNA: 3'- -CC-ACC-----UCGCCGCgCaGA-UCGCGGUUG- -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 75700 | 0.67 | 0.777788 |
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Target: 5'- --aGGAcGgGuGCGcCGUC-AGCGCCAGCg -3' miRNA: 3'- ccaCCU-CgC-CGC-GCAGaUCGCGGUUG- -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 119827 | 0.67 | 0.777788 |
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Target: 5'- uGUGGGGCGcG-GCGUCc-GCGcCCAGCc -3' miRNA: 3'- cCACCUCGC-CgCGCAGauCGC-GGUUG- -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 35275 | 0.67 | 0.774163 |
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Target: 5'- gGGUGG-GCGGgGCGggccggggacgGGCGCgGGCc -3' miRNA: 3'- -CCACCuCGCCgCGCaga--------UCGCGgUUG- -5' |
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| 4025 | 3' | -58.6 | NC_001650.1 | + | 148045 | 1.12 | 0.001261 |
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Target: 5'- cGGUGGAGCGGCGCGUCUAGCGCCAACg -3' miRNA: 3'- -CCACCUCGCCGCGCAGAUCGCGGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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