Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 403 | 5' | -58.1 | AC_000011.1 | + | 34461 | 0.66 | 0.425619 |
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Target: 5'- uGUCCUGCaAGAGUUCCC-CGuGCccuGgGAu -3' miRNA: 3'- -UAGGACG-UCUCGAGGGaGC-CGu--UgCU- -5' |
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| 403 | 5' | -58.1 | AC_000011.1 | + | 27871 | 0.66 | 0.415906 |
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Target: 5'- -aCCU-CGGAGCUCaCCUcCGGUGugGGu -3' miRNA: 3'- uaGGAcGUCUCGAG-GGA-GCCGUugCU- -5' |
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| 403 | 5' | -58.1 | AC_000011.1 | + | 22542 | 0.66 | 0.386676 |
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Target: 5'- gGUCCcgUGCAGgcaccgcAGCUugCCCUCGGCcuCGGu -3' miRNA: 3'- -UAGG--ACGUC-------UCGA--GGGAGCCGuuGCU- -5' |
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| 403 | 5' | -58.1 | AC_000011.1 | + | 1677 | 0.67 | 0.360587 |
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Target: 5'- -aCCUGUGGAGauuCUgCUUCGGUGGCGAc -3' miRNA: 3'- uaGGACGUCUC---GAgGGAGCCGUUGCU- -5' |
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| 403 | 5' | -58.1 | AC_000011.1 | + | 876 | 0.67 | 0.350156 |
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Target: 5'- aGUCgCUGUcugagcuAGAGCUCgaagccuCCUCGGCAGCu- -3' miRNA: 3'- -UAG-GACG-------UCUCGAG-------GGAGCCGUUGcu -5' |
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| 403 | 5' | -58.1 | AC_000011.1 | + | 22303 | 0.69 | 0.252425 |
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Target: 5'- -gCCUGCuGggccuuGGCUCCCUCGGUGAaGAa -3' miRNA: 3'- uaGGACGuC------UCGAGGGAGCCGUUgCU- -5' |
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| 403 | 5' | -58.1 | AC_000011.1 | + | 31560 | 0.69 | 0.245165 |
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Target: 5'- cAUCCUGCAGcuccuugacGGCUCUCUCcugugcuGGCGaauGCGAa -3' miRNA: 3'- -UAGGACGUC---------UCGAGGGAG-------CCGU---UGCU- -5' |
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| 403 | 5' | -58.1 | AC_000011.1 | + | 23084 | 0.73 | 0.145641 |
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Target: 5'- gGUCUUGCGGGGUUUCUucuugggCGGCAGCGGc -3' miRNA: 3'- -UAGGACGUCUCGAGGGa------GCCGUUGCU- -5' |
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| 403 | 5' | -58.1 | AC_000011.1 | + | 20010 | 1.06 | 0.000383 |
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Target: 5'- gAUCCUGCAGAGCUCCCUCGGCAACGAc -3' miRNA: 3'- -UAGGACGUCUCGAGGGAGCCGUUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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