miRNA: 3'- -CCGCCUCUu---UAUGGGaGA------GGU--CCGUC- -5'
miRNA: 3'- -CCgCCUCUUUa----UGGG-----------AGA--------GGUCCGUC- -5'
miRNA: 3'- -CCgCCU--CUUUA-UGGG-AGAGGUCCGUC- -5'
Results 101 - 103 of 103 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 4036 | 5' | -54.4 | NC_001650.1 | + | 180526 | 0.7 | 0.81526 |
|
Target: 5'- cGGCGGGGAccauGUGCCC-CUuaagggCCAauGGCAGa -3' miRNA: 3'- -CCGCCUCUu---UAUGGGaGA------GGU--CCGUC- -5' |
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| 4036 | 5' | -54.4 | NC_001650.1 | + | 181775 | 0.67 | 0.94288 |
|
Target: 5'- uGGgGGGGAAGggacgGCCCcgcccgcuuccUCUuuacagacCCGGGCAGg -3' miRNA: 3'- -CCgCCUCUUUa----UGGG-----------AGA--------GGUCCGUC- -5' |
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| 4036 | 5' | -54.4 | NC_001650.1 | + | 183555 | 0.79 | 0.351471 |
|
Target: 5'- uGGgGGAguGAGGUcACCCgUCUCCGGGCAGa -3' miRNA: 3'- -CCgCCU--CUUUA-UGGG-AGAGGUCCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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