miRNA: 3'- -CCGCCUCU-----UUAUgGGAG-------AGGUCCGUC- -5'
miRNA: 3'- -CCGCCU--CuuUAuGGGAGaGgUCCguC- -5'
miRNA: 3'- ccGCCUCUUuaUGGGAgaGGUCCGUc -5'
Results 101 - 103 of 103 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 4036 | 5' | -54.4 | NC_001650.1 | + | 6606 | 0.68 | 0.922269 |
|
Target: 5'- uGGUGGAGGgugguGGUGgUCUCugagguuUCCAGGUAGa -3' miRNA: 3'- -CCGCCUCU-----UUAUgGGAG-------AGGUCCGUC- -5' |
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| 4036 | 5' | -54.4 | NC_001650.1 | + | 5041 | 0.69 | 0.885639 |
|
Target: 5'- aGGCGGAcgGggGU-CCCUC-CgGGGguGg -3' miRNA: 3'- -CCGCCU--CuuUAuGGGAGaGgUCCguC- -5' |
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| 4036 | 5' | -54.4 | NC_001650.1 | + | 4076 | 0.67 | 0.938221 |
|
Target: 5'- -aUGGAGAG--GCCCUg-CCAGGCAu -3' miRNA: 3'- ccGCCUCUUuaUGGGAgaGGUCCGUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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