Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 405 | 3' | -59.7 | AC_000011.1 | + | 28269 | 0.67 | 0.371909 |
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Target: 5'- cCCCGGUGcUGA-CGGCUCCccGCGCa -3' miRNA: 3'- -GGGCCGUuGCUgGCCGAGGacUGCGg -5' |
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| 405 | 3' | -59.7 | AC_000011.1 | + | 15569 | 0.67 | 0.354972 |
|
Target: 5'- cCCCGuGCGcgugcGCGGgcacuuCCGcGCUCCcuggGGCGCCc -3' miRNA: 3'- -GGGC-CGU-----UGCU------GGC-CGAGGa---CUGCGG- -5' |
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| 405 | 3' | -59.7 | AC_000011.1 | + | 9035 | 0.67 | 0.354972 |
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Target: 5'- aUCCaGCGGCGGagCGGCaucUCgCUGACGUCg -3' miRNA: 3'- -GGGcCGUUGCUg-GCCG---AG-GACUGCGG- -5' |
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| 405 | 3' | -59.7 | AC_000011.1 | + | 12994 | 0.67 | 0.354972 |
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Target: 5'- --aGaGCGugGGCCuGUUCCUGAUGCa -3' miRNA: 3'- gggC-CGUugCUGGcCGAGGACUGCGg -5' |
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| 405 | 3' | -59.7 | AC_000011.1 | + | 15772 | 0.67 | 0.354139 |
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Target: 5'- gCCGGCGGCGGcgcaucgcCCGGCggcaCCgGAgcaccccCGCCa -3' miRNA: 3'- gGGCCGUUGCU--------GGCCGa---GGaCU-------GCGG- -5' |
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| 405 | 3' | -59.7 | AC_000011.1 | + | 10145 | 0.67 | 0.354139 |
|
Target: 5'- -gCGGCGGCGGCUGGCgguagagcggccaUCgCUcgguggcgggGGCGCCg -3' miRNA: 3'- ggGCCGUUGCUGGCCG-------------AG-GA----------CUGCGG- -5' |
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| 405 | 3' | -59.7 | AC_000011.1 | + | 20430 | 1.12 | 0.000168 |
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Target: 5'- gCCCGGCAACGACCGGCUCCUGACGCCc -3' miRNA: 3'- -GGGCCGUUGCUGGCCGAGGACUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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