Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 406 | 3' | -62 | AC_000011.1 | + | 3060 | 0.66 | 0.258761 |
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Target: 5'- aCGGuGGCCAGCauauggcuguUCCCGCCGGCg- -3' miRNA: 3'- -GUC-CCGGUCGggau------GGGGCGGUUGau -5' |
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| 406 | 3' | -62 | AC_000011.1 | + | 22067 | 0.67 | 0.223597 |
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Target: 5'- uCAGGGCCgGGUgCUucACgCUCGCCAGCa- -3' miRNA: 3'- -GUCCCGG-UCGgGA--UG-GGGCGGUUGau -5' |
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| 406 | 3' | -62 | AC_000011.1 | + | 26792 | 0.67 | 0.223597 |
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Target: 5'- aCAGGG-UGGCgCCg--CCCGCCAGCUGg -3' miRNA: 3'- -GUCCCgGUCG-GGaugGGGCGGUUGAU- -5' |
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| 406 | 3' | -62 | AC_000011.1 | + | 3592 | 0.68 | 0.175638 |
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Target: 5'- --cGGCCGGCCCgUGCagCCCGCgAACUc -3' miRNA: 3'- gucCCGGUCGGG-AUG--GGGCGgUUGAu -5' |
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| 406 | 3' | -62 | AC_000011.1 | + | 20488 | 0.69 | 0.161806 |
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Target: 5'- uCAGGaGCCGGUCgUugCCgGgCCAGCUGa -3' miRNA: 3'- -GUCC-CGGUCGGgAugGGgC-GGUUGAU- -5' |
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| 406 | 3' | -62 | AC_000011.1 | + | 20179 | 0.7 | 0.133283 |
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Target: 5'- uCGGcGGCCAacauGCUCUACCCCaucccgGCCAACg- -3' miRNA: 3'- -GUC-CCGGU----CGGGAUGGGG------CGGUUGau -5' |
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| 406 | 3' | -62 | AC_000011.1 | + | 11040 | 0.72 | 0.092271 |
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Target: 5'- cCAGGcgcGCCAGCCCcucGCCCUcuuccaagGCCAGCUGg -3' miRNA: 3'- -GUCC---CGGUCGGGa--UGGGG--------CGGUUGAU- -5' |
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| 406 | 3' | -62 | AC_000011.1 | + | 34211 | 0.8 | 0.020713 |
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Target: 5'- aCAGGGCCAGCaCC-GCCCCGCCcGCc- -3' miRNA: 3'- -GUCCCGGUCG-GGaUGGGGCGGuUGau -5' |
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| 406 | 3' | -62 | AC_000011.1 | + | 20748 | 1.05 | 0.000182 |
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Target: 5'- cCAGGGCCAGCCCUACCCCGCCAACUAc -3' miRNA: 3'- -GUCCCGGUCGGGAUGGGGCGGUUGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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