Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 407 | 5' | -57 | AC_000011.1 | + | 23612 | 0.66 | 0.466704 |
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Target: 5'- ---cUGCCAGCUCCuccucguGCuCCGCGgGCg- -3' miRNA: 3'- agauACGGUUGAGG-------CG-GGUGCgCGau -5' |
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| 407 | 5' | -57 | AC_000011.1 | + | 10990 | 0.66 | 0.45742 |
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Target: 5'- cUCUGU-CCAGCcCCGCUCACG-GCg- -3' miRNA: 3'- -AGAUAcGGUUGaGGCGGGUGCgCGau -5' |
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| 407 | 5' | -57 | AC_000011.1 | + | 3536 | 0.66 | 0.45742 |
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Target: 5'- ----aGCCGcUUCCGCUCAUGCuGCUGc -3' miRNA: 3'- agauaCGGUuGAGGCGGGUGCG-CGAU- -5' |
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| 407 | 5' | -57 | AC_000011.1 | + | 17939 | 0.66 | 0.447221 |
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Target: 5'- aCUGggucUGCCcACcacgCgGCCCACGCGCc- -3' miRNA: 3'- aGAU----ACGGuUGa---GgCGGGUGCGCGau -5' |
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| 407 | 5' | -57 | AC_000011.1 | + | 8262 | 0.68 | 0.344156 |
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Target: 5'- cCUGga-CAGCUCCgGCCUGCGCGCc- -3' miRNA: 3'- aGAUacgGUUGAGG-CGGGUGCGCGau -5' |
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| 407 | 5' | -57 | AC_000011.1 | + | 15956 | 0.7 | 0.280618 |
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Target: 5'- ----cGCCAGCaugUCCGCCCGCG-GCg- -3' miRNA: 3'- agauaCGGUUG---AGGCGGGUGCgCGau -5' |
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| 407 | 5' | -57 | AC_000011.1 | + | 4561 | 0.7 | 0.259311 |
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Target: 5'- gUCUuUGCCcaGGCcgCCGCCCACGgGCc- -3' miRNA: 3'- -AGAuACGG--UUGa-GGCGGGUGCgCGau -5' |
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| 407 | 5' | -57 | AC_000011.1 | + | 3889 | 0.74 | 0.149303 |
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Target: 5'- gCUggGUCAGCU-CGCCCAgGCGCUGg -3' miRNA: 3'- aGAuaCGGUUGAgGCGGGUgCGCGAU- -5' |
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| 407 | 5' | -57 | AC_000011.1 | + | 20914 | 1.07 | 0.000439 |
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Target: 5'- cUCUAUGCCAACUCCGCCCACGCGCUAg -3' miRNA: 3'- -AGAUACGGUUGAGGCGGGUGCGCGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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