Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 412 | 3' | -61.6 | AC_000011.1 | + | 18213 | 0.66 | 0.280843 |
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Target: 5'- cGGCGCuucaCACUC--UGCACUCCCAGa- -3' miRNA: 3'- -UCGCGc---GUGGGccACGUGAGGGUCaa -5' |
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| 412 | 3' | -61.6 | AC_000011.1 | + | 15517 | 0.67 | 0.228351 |
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Target: 5'- uGCGCGCGCCCagcaagauguacGGagGCGCUCgCCAa-- -3' miRNA: 3'- uCGCGCGUGGG------------CCa-CGUGAG-GGUcaa -5' |
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| 412 | 3' | -61.6 | AC_000011.1 | + | 16017 | 0.67 | 0.210913 |
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Target: 5'- uGCGCGUGCCC-GUGCGCaCCCGc-- -3' miRNA: 3'- uCGCGCGUGGGcCACGUGaGGGUcaa -5' |
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| 412 | 3' | -61.6 | AC_000011.1 | + | 23299 | 0.68 | 0.20536 |
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Target: 5'- gGGUGCGCuCCCGGcgGCGCUCUg---- -3' miRNA: 3'- -UCGCGCGuGGGCCa-CGUGAGGgucaa -5' |
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| 412 | 3' | -61.6 | AC_000011.1 | + | 15574 | 0.68 | 0.199934 |
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Target: 5'- uGCGCGUGCgCGG-GCACUUCCGc-- -3' miRNA: 3'- uCGCGCGUGgGCCaCGUGAGGGUcaa -5' |
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| 412 | 3' | -61.6 | AC_000011.1 | + | 16059 | 0.69 | 0.156505 |
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Target: 5'- -aCGCGCACaCCGGUggcggcgucGCGCaCCCAGUa -3' miRNA: 3'- ucGCGCGUG-GGCCA---------CGUGaGGGUCAa -5' |
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| 412 | 3' | -61.6 | AC_000011.1 | + | 18083 | 0.72 | 0.097076 |
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Target: 5'- cGCGCGCgACCCGGggGCACcgCCCGc-- -3' miRNA: 3'- uCGCGCG-UGGGCCa-CGUGa-GGGUcaa -5' |
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| 412 | 3' | -61.6 | AC_000011.1 | + | 22437 | 0.72 | 0.091681 |
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Target: 5'- cGCGCGCugCUGcGUGCACgacgCCgGGUg -3' miRNA: 3'- uCGCGCGugGGC-CACGUGa---GGgUCAa -5' |
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| 412 | 3' | -61.6 | AC_000011.1 | + | 22596 | 1.04 | 0.000258 |
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Target: 5'- cAGCGCGCACCCGGUGCACUCCCAGUUc -3' miRNA: 3'- -UCGCGCGUGGGCCACGUGAGGGUCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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