Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 412 | 5' | -52.9 | AC_000011.1 | + | 15505 | 0.67 | 0.693638 |
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Target: 5'- cCGGUUgGGGccUGCGCGcgcccagcaagaUGUACGGAGg -3' miRNA: 3'- -GCUAGaCCCuuACGCGC------------ACGUGCUUC- -5' |
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| 412 | 5' | -52.9 | AC_000011.1 | + | 18148 | 0.68 | 0.579015 |
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Target: 5'- gGGUCUGGGAGUGCagaGUGU--GAAGc -3' miRNA: 3'- gCUAGACCCUUACGcg-CACGugCUUC- -5' |
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| 412 | 5' | -52.9 | AC_000011.1 | + | 30615 | 0.69 | 0.533912 |
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Target: 5'- gCGcgUUGGGAAUGCGgCG-GCA-GAAGa -3' miRNA: 3'- -GCuaGACCCUUACGC-GCaCGUgCUUC- -5' |
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| 412 | 5' | -52.9 | AC_000011.1 | + | 17358 | 0.7 | 0.511821 |
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Target: 5'- gCGAUgaUGGGGAUcaGCGCGgGCAgGAAGc -3' miRNA: 3'- -GCUAg-ACCCUUA--CGCGCaCGUgCUUC- -5' |
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| 412 | 5' | -52.9 | AC_000011.1 | + | 22659 | 0.7 | 0.490118 |
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Target: 5'- aGAaCUGGGAGUGCaccGgGUGCGCGc-- -3' miRNA: 3'- gCUaGACCCUUACG---CgCACGUGCuuc -5' |
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| 412 | 5' | -52.9 | AC_000011.1 | + | 22630 | 1.08 | 0.001174 |
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Target: 5'- gCGAUCUGGGAAUGCGCGUGCACGAAGc -3' miRNA: 3'- -GCUAGACCCUUACGCGCACGUGCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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