Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4143 | 3' | -51.7 | NC_001664.1 | + | 1961 | 0.68 | 0.954473 |
Target: 5'- gGGGCGUGCGGAGAgaUGGAc------ -3' miRNA: 3'- -UCUGCACGCCUCUacACCUcucuuug -5' |
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4143 | 3' | -51.7 | NC_001664.1 | + | 152724 | 1.11 | 0.005631 |
Target: 5'- cAGACGUGCGGAGAUGUGGAGAGAAACg -3' miRNA: 3'- -UCUGCACGCCUCUACACCUCUCUUUG- -5' |
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4143 | 3' | -51.7 | NC_001664.1 | + | 1642 | 0.99 | 0.033766 |
Target: 5'- cAGAC-UGCGGAGAUGUGGAGAGAAACg -3' miRNA: 3'- -UCUGcACGCCUCUACACCUCUCUUUG- -5' |
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4143 | 3' | -51.7 | NC_001664.1 | + | 155805 | 0.71 | 0.873617 |
Target: 5'- --cUGUGaCGGGGAgggGUGGGGAGAuaAACa -3' miRNA: 3'- ucuGCAC-GCCUCUa--CACCUCUCU--UUG- -5' |
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4143 | 3' | -51.7 | NC_001664.1 | + | 154548 | 0.71 | 0.873617 |
Target: 5'- cGACucgaGCGGGGGUG-GGAGGGAAGg -3' miRNA: 3'- uCUGca--CGCCUCUACaCCUCUCUUUg -5' |
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4143 | 3' | -51.7 | NC_001664.1 | + | 4723 | 0.71 | 0.873617 |
Target: 5'- --cUGUGaCGGGGAgggGUGGGGAGAuaAACa -3' miRNA: 3'- ucuGCAC-GCCUCUa--CACCUCUCU--UUG- -5' |
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4143 | 3' | -51.7 | NC_001664.1 | + | 3467 | 0.71 | 0.873617 |
Target: 5'- cGACucgaGCGGGGGUG-GGAGGGAAGg -3' miRNA: 3'- uCUGca--CGCCUCUACaCCUCUCUUUg -5' |
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4143 | 3' | -51.7 | NC_001664.1 | + | 153581 | 0.7 | 0.901694 |
Target: 5'- gGGACGgGCGGAGcgGaGGGGcgcGAGACa -3' miRNA: 3'- -UCUGCaCGCCUCuaCaCCUCu--CUUUG- -5' |
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4143 | 3' | -51.7 | NC_001664.1 | + | 2500 | 0.7 | 0.901694 |
Target: 5'- gGGACGgGCGGAGcgGaGGGGcgcGAGACa -3' miRNA: 3'- -UCUGCaCGCCUCuaCaCCUCu--CUUUG- -5' |
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4143 | 3' | -51.7 | NC_001664.1 | + | 153043 | 0.68 | 0.954473 |
Target: 5'- gGGGCGUGCGGAGAgaUGGAc------ -3' miRNA: 3'- -UCUGCACGCCUCUacACCUcucuuug -5' |
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4143 | 3' | -51.7 | NC_001664.1 | + | 154654 | 0.68 | 0.958004 |
Target: 5'- cGGCGUGUgugcuccggggagGGGGAUG-GGAGGGGuugGACc -3' miRNA: 3'- uCUGCACG-------------CCUCUACaCCUCUCU---UUG- -5' |
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4143 | 3' | -51.7 | NC_001664.1 | + | 3573 | 0.68 | 0.958004 |
Target: 5'- cGGCGUGUgugcuccggggagGGGGAUG-GGAGGGGuugGACc -3' miRNA: 3'- uCUGCACG-------------CCUCUACaCCUCUCU---UUG- -5' |
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4143 | 3' | -51.7 | NC_001664.1 | + | 154148 | 0.67 | 0.97708 |
Target: 5'- gGGGCGauaUGCGGAGGUugucagcgGUGGuGgcGGAGGCg -3' miRNA: 3'- -UCUGC---ACGCCUCUA--------CACCuC--UCUUUG- -5' |
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4143 | 3' | -51.7 | NC_001664.1 | + | 3066 | 0.67 | 0.97708 |
Target: 5'- gGGGCGauaUGCGGAGGUugucagcgGUGGuGgcGGAGGCg -3' miRNA: 3'- -UCUGC---ACGCCUCUA--------CACCuC--UCUUUG- -5' |
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4143 | 3' | -51.7 | NC_001664.1 | + | 153677 | 0.66 | 0.987189 |
Target: 5'- gAGACGggagaggGCGGAGAcaacGgagcgGGAGAGuagGACg -3' miRNA: 3'- -UCUGCa------CGCCUCUa---Ca----CCUCUCu--UUG- -5' |
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4143 | 3' | -51.7 | NC_001664.1 | + | 2596 | 0.66 | 0.987189 |
Target: 5'- gAGACGggagaggGCGGAGAcaacGgagcgGGAGAGuagGACg -3' miRNA: 3'- -UCUGCa------CGCCUCUa---Ca----CCUCUCu--UUG- -5' |
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4143 | 3' | -51.7 | NC_001664.1 | + | 92934 | 0.73 | 0.778909 |
Target: 5'- cGACuuGUGUGGAGAUGUacugGGAGAGuauGCu -3' miRNA: 3'- uCUG--CACGCCUCUACA----CCUCUCuu-UG- -5' |
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4143 | 3' | -51.7 | NC_001664.1 | + | 157814 | 0.66 | 0.989961 |
Target: 5'- gAGACGUGacCGGAGAaaaucaugcauugUGUGGGGuGuguauGCg -3' miRNA: 3'- -UCUGCAC--GCCUCU-------------ACACCUCuCuu---UG- -5' |
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4143 | 3' | -51.7 | NC_001664.1 | + | 6733 | 0.66 | 0.989961 |
Target: 5'- gAGACGUGacCGGAGAaaaucaugcauugUGUGGGGuGuguauGCg -3' miRNA: 3'- -UCUGCAC--GCCUCU-------------ACACCUCuCuu---UG- -5' |
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4143 | 3' | -51.7 | NC_001664.1 | + | 74175 | 0.67 | 0.97708 |
Target: 5'- -uGCGUGUGGAGuUGaagaucuuuaUGGAGGGAAu- -3' miRNA: 3'- ucUGCACGCCUCuAC----------ACCUCUCUUug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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