Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4143 | 5' | -59.5 | NC_001664.1 | + | 152690 | 1.02 | 0.003014 |
Target: 5'- gAAGCCAUCCGCCCUCUCCGCACAGUUc -3' miRNA: 3'- -UUCGGUAGGCGGGAGAGGCGUGUCAA- -5' |
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4143 | 5' | -59.5 | NC_001664.1 | + | 1609 | 1.02 | 0.003014 |
Target: 5'- gAAGCCAUCCGCCCUCUCCGCACAGUUc -3' miRNA: 3'- -UUCGGUAGGCGGGAGAGGCGUGUCAA- -5' |
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4143 | 5' | -59.5 | NC_001664.1 | + | 2210 | 0.74 | 0.288777 |
Target: 5'- cGAGCCguccGUCCGCCCUCggugUCCGC-CAGg- -3' miRNA: 3'- -UUCGG----UAGGCGGGAG----AGGCGuGUCaa -5' |
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4143 | 5' | -59.5 | NC_001664.1 | + | 153292 | 0.74 | 0.288777 |
Target: 5'- cGAGCCguccGUCCGCCCUCggugUCCGC-CAGg- -3' miRNA: 3'- -UUCGG----UAGGCGGGAG----AGGCGuGUCaa -5' |
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4143 | 5' | -59.5 | NC_001664.1 | + | 154168 | 0.69 | 0.504446 |
Target: 5'- --aCUAUCCGCCCUCcCCgaGCACGGUc -3' miRNA: 3'- uucGGUAGGCGGGAGaGG--CGUGUCAa -5' |
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4143 | 5' | -59.5 | NC_001664.1 | + | 3086 | 0.69 | 0.504446 |
Target: 5'- --aCUAUCCGCCCUCcCCgaGCACGGUc -3' miRNA: 3'- uucGGUAGGCGGGAGaGG--CGUGUCAa -5' |
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4143 | 5' | -59.5 | NC_001664.1 | + | 2533 | 0.67 | 0.675762 |
Target: 5'- -cGCCGg-CGCCCUCgUCCGCGCcGg- -3' miRNA: 3'- uuCGGUagGCGGGAG-AGGCGUGuCaa -5' |
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4143 | 5' | -59.5 | NC_001664.1 | + | 153615 | 0.67 | 0.675762 |
Target: 5'- -cGCCGg-CGCCCUCgUCCGCGCcGg- -3' miRNA: 3'- uuCGGUagGCGGGAG-AGGCGUGuCaa -5' |
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4143 | 5' | -59.5 | NC_001664.1 | + | 8360 | 0.67 | 0.655397 |
Target: 5'- -cGCCGUCCGCCCcuauaccgUCgUCCGCucuuuCGGg- -3' miRNA: 3'- uuCGGUAGGCGGG--------AG-AGGCGu----GUCaa -5' |
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4143 | 5' | -59.5 | NC_001664.1 | + | 10519 | 0.66 | 0.735716 |
Target: 5'- cGAGCCugcgCCGCgCCUCgUCGCACAu-- -3' miRNA: 3'- -UUCGGua--GGCG-GGAGaGGCGUGUcaa -5' |
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4143 | 5' | -59.5 | NC_001664.1 | + | 9011 | 0.66 | 0.70602 |
Target: 5'- -uGCCcgCCGUCUaucaacuuUCUCCGCACAu-- -3' miRNA: 3'- uuCGGuaGGCGGG--------AGAGGCGUGUcaa -5' |
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4143 | 5' | -59.5 | NC_001664.1 | + | 77191 | 0.66 | 0.695985 |
Target: 5'- -uGCCAUCCGCaaggaUUCUUCGguUAGUUu -3' miRNA: 3'- uuCGGUAGGCGg----GAGAGGCguGUCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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