Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
4144 | 3' | -61.8 | NC_001664.1 | + | 8598 | 0.66 | 0.731015 |
Target: 5'- cGGGGGu-CUGCaaaaauGUCUGCGGAaaggucaaccgCCCGGc -3' miRNA: 3'- -CCCCCuuGGCG------CAGACGCCUg----------GGGCU- -5' |
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4144 | 3' | -61.8 | NC_001664.1 | + | 2695 | 0.66 | 0.702831 |
Target: 5'- cGGcGGGAacGCCGCGggaUGCccggaGGACaCCCGc -3' miRNA: 3'- -CC-CCCU--UGGCGCag-ACG-----CCUG-GGGCu -5' |
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4144 | 3' | -61.8 | NC_001664.1 | + | 153777 | 0.66 | 0.702831 |
Target: 5'- cGGcGGGAacGCCGCGggaUGCccggaGGACaCCCGc -3' miRNA: 3'- -CC-CCCU--UGGCGCag-ACG-----CCUG-GGGCu -5' |
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4144 | 3' | -61.8 | NC_001664.1 | + | 4552 | 0.67 | 0.654893 |
Target: 5'- cGGcGGACCGCGguguuuUCUGCGGAa-CCGAc -3' miRNA: 3'- cCCcCUUGGCGC------AGACGCCUggGGCU- -5' |
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4144 | 3' | -61.8 | NC_001664.1 | + | 155633 | 0.67 | 0.654893 |
Target: 5'- cGGcGGACCGCGguguuuUCUGCGGAa-CCGAc -3' miRNA: 3'- cCCcCUUGGCGC------AGACGCCUggGGCU- -5' |
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4144 | 3' | -61.8 | NC_001664.1 | + | 62904 | 0.68 | 0.587275 |
Target: 5'- cGGuGuuuCUGuCGUCUGUGGAUCCCGAa -3' miRNA: 3'- cCCcCuu-GGC-GCAGACGCCUGGGGCU- -5' |
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4144 | 3' | -61.8 | NC_001664.1 | + | 30693 | 0.7 | 0.46581 |
Target: 5'- cGGaGGAAUgggaaggUGCGUCUGCGG-CUCCGGa -3' miRNA: 3'- cCC-CCUUG-------GCGCAGACGCCuGGGGCU- -5' |
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4144 | 3' | -61.8 | NC_001664.1 | + | 2421 | 0.72 | 0.375434 |
Target: 5'- uGGGGGGuuccuCCGCGggacCUgGCGGACaCCGAg -3' miRNA: 3'- -CCCCCUu----GGCGCa---GA-CGCCUGgGGCU- -5' |
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4144 | 3' | -61.8 | NC_001664.1 | + | 153502 | 0.72 | 0.375434 |
Target: 5'- uGGGGGGuuccuCCGCGggacCUgGCGGACaCCGAg -3' miRNA: 3'- -CCCCCUu----GGCGCa---GA-CGCCUGgGGCU- -5' |
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4144 | 3' | -61.8 | NC_001664.1 | + | 9160 | 0.74 | 0.297023 |
Target: 5'- cGGGGGAGCCGUGg-UGCGucuCUCCGAu -3' miRNA: 3'- -CCCCCUUGGCGCagACGCcu-GGGGCU- -5' |
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4144 | 3' | -61.8 | NC_001664.1 | + | 4507 | 1.1 | 0.000947 |
Target: 5'- cGGGGGAACCGCGUCUGCGGACCCCGAu -3' miRNA: 3'- -CCCCCUUGGCGCAGACGCCUGGGGCU- -5' |
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4144 | 3' | -61.8 | NC_001664.1 | + | 155588 | 1.1 | 0.000947 |
Target: 5'- cGGGGGAACCGCGUCUGCGGACCCCGAu -3' miRNA: 3'- -CCCCCUUGGCGCAGACGCCUGGGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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