miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
4145 3' -55.3 NC_001664.1 + 2806 0.67 0.870564
Target:  5'- -cGCCGUguguGCGGc--GGGCGGCUgugacuUGCCg -3'
miRNA:   3'- auCGGCA----UGUCaaaCCUGCCGG------ACGG- -5'
4145 3' -55.3 NC_001664.1 + 5019 1.11 0.002273
Target:  5'- aUAGCCGUACAGUUUGGACGGCCUGCCg -3'
miRNA:   3'- -AUCGGCAUGUCAAACCUGCCGGACGG- -5'
4145 3' -55.3 NC_001664.1 + 84970 0.66 0.933328
Target:  5'- uUAGCgGUugAGacgUGuGACGuGCCUGUUg -3'
miRNA:   3'- -AUCGgCAugUCaa-AC-CUGC-CGGACGG- -5'
4145 3' -55.3 NC_001664.1 + 153888 0.67 0.870564
Target:  5'- -cGCCGUguguGCGGc--GGGCGGCUgugacuUGCCg -3'
miRNA:   3'- auCGGCA----UGUCaaaCCUGCCGG------ACGG- -5'
4145 3' -55.3 NC_001664.1 + 156101 1.11 0.002273
Target:  5'- aUAGCCGUACAGUUUGGACGGCCUGCCg -3'
miRNA:   3'- -AUCGGCAUGUCAAACCUGCCGGACGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.